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Volume 72, Issue 10

sp. nov., isolated from paddy soil No Access

Abstract

A polyphasic taxonomic approach was used to characterize a Gram-stain-negative, orange-coloured bacterium (designated strain CC-SYL272) isolated from paddy soil. Cells were observed to be strictly aerobic, non-motile and non-spore-forming rods, exhibiting positive catalase and oxidase. Strain CC-SYL272 was found to grow optimally at 20–40 °C, pH 6.0–8.0 and NaCl 0–2 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CC-SYL272 belongs to the genus , family , and is most closely related to (97.8 %) followed by (97.2 %) and established a distinct taxonomic lineage associated with these species. The highest orthologous average nucleotide identity (OrthoANI) values were recorded for strain CC-SYL272 species (69.1–83.5 %, =8). The mean digital DNA–DNA hybridization (dDDH) value obtained for strain CC-SYL272 against was 27.3 %. The polar lipid profile consisted of phosphatidylethanolamine and five unidentified lipids. The major polyamines were putrescine and -homospermidine. The dominating cellular fatty acids (>5 %) included iso-C, iso-C G, iso-C 3OH and C 6/C 7. The draft genome (6.25 Mb) of strain CC-SYL272 spanned three contigs having 47.1 mol% DNA G+C content, 5087 protein-encoding genes, 10 rRNA genes and 44 tRNA genes. The genome harboured genes involved in the depolymerization of both animal and plant polysaccharides. Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence, OrthoANI, dDDH and the phylogenomic placement, strain CC-SYL272 is considered to represent a novel species of the genus , affiliated to the family , for which the name sp. nov. is proposed. The type strain is CC-SYL272 (=BCRC 81319=JCM 34758).

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  • Accepted:
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Keyword(s): Niabella agricola , orthologous average nucleotide identity (OrthoANI) and polyphasic taxonomic approach
© 2022 The Authors
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2022-10-19
2024-05-07
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Niabella agricola sp. nov., isolated from paddy soil
Int J Syst Evol Microbiol 72, 005559 (2022); https://doi.org/10.1099/ijsem.0.005559
/content/journal/ijsem/10.1099/ijsem.0.005559
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