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Detection of Brugia malayi in laboratory and wild-caught Mansonioides mosquitoes (Diptera: Culicidae) using Hha I PCR assay

Published online by Cambridge University Press:  09 March 2007

S.L. Hoti ,
V. Vasuki ,
M.W. Lizotte ,
K.P. Patra ,
G. Ravi ,
P. Vanamail ,
A. Manonmani ,
S. Sabesan ,
K. Krishnamoorthy  and
S.A. Williams
S.L. Hoti*
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
V. Vasuki
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
M.W. Lizotte
Affiliation:
Clark Science Centre, Smith College, Northampton, USA
K.P. Patra
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
G. Ravi
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
P. Vanamail
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
A. Manonmani
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
S. Sabesan
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
K. Krishnamoorthy
Affiliation:
Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India
S.A. Williams
Affiliation:
Clark Science Centre, Smith College, Northampton, USA
*
*Fax: 0413-372422, 372041 E-mail: mosquito@md5.vsnl.net.in
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Abstract

An Hha 1 based polymerase chain reaction (PCR) assay developed for the detection of Brugia malayi, the causative agent of Brugian lymphatic filariasis, was evaluated for its sensitivity in the laboratory and for its usefulness in measuring changes in transmission of the disease in the field. Laboratory studies showed that the new assay was highly sensitive in comparison with the standard dissection and microscopy technique. The assay can detect as little as 4 pg of parasite DNA or a single microfilaria in pools of up to 100 mosquitoes. The optimum pool size for convenience was found to be 50 mosquitoes per pool. The efficacy of PCR assay was evaluated in filariasis control programmes in operation in endemic areas of Kerala State, South India. The infection rates obtained by the Hha I PCR assay and the conventional dissection and microscopy technique were 1.2% and 1.7% respectively in operational areas and 8.3% and 4.4% respectively, in check areas, which were not significantly different (P < 0.05). Thus, the Hha I PCR assay was found to be as sensitive as the conventional technique and hence it can be used as a new epidemiological tool for assessing parasite infection in field-collected mosquitoes.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 91 , Issue 2 , April 2001 , pp. 87 - 92
Copyright
Copyright © Cambridge University Press 2001

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References

Chanteau, S., Luguiaud, P., Failloux, A.B. & Williams, S. (1994) Detection of Wuchereria bancrofti larvae in pooled mosquitoes by the polymerase chain reaction. Transactions of the Royal Society of Tropical Medicine and Hygiene 89, 665–666.CrossRefGoogle Scholar
Charles, H.H. (1987) Epidemiology in medicine. 331 pp. Little, Brown & Co.Google Scholar
Iyengar, M.O.T. (1938) Studies on the epidemiology of filariasis in Travancore. Indian Medical Research Memoirs 30, 1–179.Google Scholar
Katholi, C.R., Toe, L., Merriweather, A. & Unnasch, T.R. (1995) Determining the prevalence of Onchocerca volvulus infection in vector populations by PCR screening of pools of blackflies. Journal of Infectious Diseases 172, 1414–1417.CrossRefGoogle Scholar
Lizotte, M.R., Supali, T., Partono, F. & Williams, S.A. (1994) A polymerase chain reaction assay for the detection of Brugia malayi in blood. American Journal of Tropical Medicine and Hygiene 51, 314–321.CrossRefGoogle ScholarPubMed
McReynolds, L.A., DeSimone, S.M. & Williams, S.A. (1986) Cloning and comparison of repeated DNA sequences from the human filarial parasite Brugia malayi and the animal parasite Brugia pahangi. Proceedings of the National Academy of Sciences, USA 83, 797–801.Google Scholar
McReynolds, L.A., Poole, C.B., Hong, Y., Williams, S.A., Partono, F. & Bradley, J. (1993) Recent advances in the application of molecular biology in filariasis. SouthEast Asian Journal of Tropical Medicine and Public Health 24, Supplement 2, 1–112.Google Scholar
Nicolas, L. & Scoles, G.A. (1997) Multiplex polymerase chain reaction for the detection of Dirofilaria immitis (Filarioidea: Onchocercidae) and Wuchereria bancrofti (Filarioidea: Dipetalonematidae) in their common vector Aedes polynesiensis (Diptera: Culicidae). Journal of Medical Entomology 34, 741–744.CrossRefGoogle ScholarPubMed
Nicolas, L., Luquiaud, P., Lardeux, F. & Mercer, D.R. (1996) A polymerase chain reaction assay to determine infection of Aedes polynesiensis by Wuchereria bancrofti. Transactions of the Royal Society of Tropical Medicine and Hygiene 90, 136–139.CrossRefGoogle ScholarPubMed
Ottesen, E.A., Duke, B.O.L., Karam, M. & Behbehani, K. (1997) Strategies and tools for the control/elimination of lymphatic filariasis. Bulletin of the World Health Organization 75, 491.Google Scholar
Paily, K.P., Gomathi, K., Hoti, S.L., Balaraman, K. & Dhanda, V. (1995) Report on the demonstration of microfilaria of Brugia malayi in the brain of an experimental animal host, Mastomys natalensis. International Journal for Parasitology 25, 1123–1126.CrossRefGoogle ScholarPubMed
Patricia, L.K.C. (1993) Current status of filariasis in SouthEast Asia. SouthEast Asian Journal of Tropical Medicine and Public Health 24, Supplement 2, 1–112.Google Scholar
Poole, C.B. & Williams, S.A. (1990) A rapid DNA assay for the identification of Brugia in blood samples. Molecular and Biochemical Parasitology 40, 129–136.CrossRefGoogle Scholar
Pradeep Kumar, N. (1990) Studies on the ecology and bionomics of adult populations of Mansonioides mosquitoes (Diptera: Culicidae) viz., Mansonia annulifera (Theobald) 1901, M. uniformis (Theobald) 1901, and M. indiana Edwards 1930, with special reference to their role in the transmission of malayan filariasis in Shertallai, Kerala State, India. PhD thesis, Pondicherry University, Pondicherry, India.Google Scholar
Pradeep Kumar, N., Sabesan, S. & Panicker, K.N. (1992) The resting and house frequenting behavior of Mansonia annulifera, M. uniformis, M. indiana, the vectors of Malayan filariasis in Kerala state, India. SouthEast Asian Journal of Tropical Medicine and Public Health 23, 324–327.Google Scholar
Raina, V.K., Singh, S.K., Ashok, Kumar & Datta, K.K. (1995) Present status of Brugia malayi filariasis and its future control strategies in India. pp. 78–85 in Proceedings of the International Symposium on vectors and vector-borne diseases. National Academy of Vector Borne Diseases.Google Scholar
Rajagopalan, P.K., Panicker, K.P. & Pani, S.P. (1989) Impact of fifty years of vector control on the prevalence of Brugia malayi in Kerala state. Indian Journal of Medical Research 89, 418–425.Google Scholar
Ramzy, R., Farid, H.A., Kamal, I.H., Ibrahim, G.H., Morsey, Z.S., Faris, R., Weil, G.J., Williams, S.A. & Gad, A.M. (1997) A polymerase chain reaction-based assay for detection of Wuchereria bancrofti in human blood and Culex pipiens. Transactions of the Royal Society of Tropical Medicine and Hygiene 91, 156–160.CrossRefGoogle ScholarPubMed
Sabesan, S., Kumar, V.P., Krishnamoorthy, K. & Panicker, K.N. (1991) Seasonal abundance and biting behavior of Mansonia annulifera, M. uniformis and M. indiana and their relative role in the transmission of brugian filariasis in Shertallai (Kerala State). Indian Journal of Medical Research 93, 253–258.Google Scholar
Sabesan, S., Pradeep Kumar, N., Rajendran, G. & Krishnamoorthy, K. (2000) The Cherthala Project: control of Brugian filariasis through integrated methods (1986–1998). Miscellaneous Publication, VCRC 23, 1–53.Google Scholar
Williams, S.A., De Simone, S.M. & McReynolds, L.A. (1988) Species-specific oligonucleotide probes for the identification of human filarial parasites. Molecular and Biochemical Parasitology 28, 163–170.CrossRefGoogle ScholarPubMed
World Health Organization (1979) Lymphatic filariasis: the disease and its control. Fifth Report of the WHO Expert Committee on filariasis. World Health Organization Technical Report Series 702, 1–71.Google Scholar
World Health OrganizationWorld Health Organization (1998) Life in the 21st century. A vision for all. World Health Organization Report, 45.Google Scholar
Zhong, M., McCarthy, J., Bierwert, L., Lizotte, W.M., Chanteau, S., Nutman, T.B., Ottesen, E.A. & Williams, S.A. (1996) A polymerase chain reaction assay for the detection of the parasite Wuchereria bancrofti in human blood samples. American Journal of Tropical Medicine and Hygiene 54, 357–363.Google Scholar