Issue 3, 2024
From the journal:

Chemical Communications

Metal-dependent activity control of a compact-sized 8–17 DNAzyme based on metal-mediated unnatural base pairing†

Yusuke Takezawa, ORCID logo *a   Lingyun Hu,a   Takahiro Nakama ORCID logo a  and  Mitsuhiko Shionoya ORCID logo *a  

* Corresponding authors

a Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: takezawa@chem.s.u-tokyo.ac.jp, shionoya@chem.s.u-tokyo.ac.jp

Abstract

A compact 8–17 DNAzyme was modified with a CuII-meditated artificial base pair to develop a metal-responsive allosteric DNAzyme. The base sequence was rationally designed based on the reported three-dimensional structure. The activity of the modified DNAzyme was enhanced 5.1-fold by the addition of one equivalent of CuII ions, showing good metal responsiveness. Since it has been challenging to modify compactly folded DNAzymes without losing their activity, this study demonstrates the utility of the metal-mediated artificial base pairing to create stimuli-responsive functional DNAs.

Graphical abstract: Metal-dependent activity control of a compact-sized 8–17 DNAzyme based on metal-mediated unnatural base pairing

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Article information

DOI
https://doi.org/10.1039/D3CC05520E
Article type
Communication
Submitted
09 Nov 2023
Accepted
01 Dec 2023
First published
04 Dec 2023

Chem. Commun., 2024,60, 288-291

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Metal-dependent activity control of a compact-sized 8–17 DNAzyme based on metal-mediated unnatural base pairing

Y. Takezawa, L. Hu, T. Nakama and M. Shionoya, Chem. Commun., 2024, 60, 288 DOI: 10.1039/D3CC05520E

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