Issue 48, 2022
From the journal:

Chemical Communications

Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies†

Yuying Du, ORCID logo a   Robin C. May ORCID logo a  and  Helen J. Cooper ORCID logo *a  

* Corresponding authors

a School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
E-mail: h.j.cooper@bham.ac.uk

Abstract

Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.

Graphical abstract: Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies

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Article information

DOI
https://doi.org/10.1039/D2CC02085H
Article type
Communication
Submitted
12 Apr 2022
Accepted
19 May 2022
First published
24 May 2022
This article is Open Access
Creative Commons BY license

Chem. Commun., 2022,58, 6857-6860

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Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies

Y. Du, R. C. May and H. J. Cooper, Chem. Commun., 2022, 58, 6857 DOI: 10.1039/D2CC02085H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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