The one-to-one coupling nanostructures of individual Au plasmonics and semiconducting MoS₂ layers are constructed with the modulation of proteins. The formation of hybrids is attributed to the confinement effect of proteins that are attached to the surfaces of layered MoS₂ seeds. From the hybrid nanostructures, enhanced photothermal effects are achieved due to the improvement of Au plasmonic-endowed antenna effects. Significantly, hot electrons triggered from the photonic radiation can rapidly transfer from Au nanoparticles to the conduction band of MoS₂ layers and thus the photothermal behaviors are subsequently investigated. Additionally, the hot electron inspired photothermal behaviors are accompanied by the electron transition from the conduction band to the valence band of MoS₂ hosts. Significantly, the mechanism of improved photothermal efficiency is ascribed to the synergistic effects. In order to identify the value of photothermal antenna effects, the heat radiation results in the escape of proteins from the interface and a clean surface of MoS₂ is obtained. The photonic-responsive interfacial modification of layered MoS₂ is promoted. Furthermore, the single-stranded DNA exhibits strong interaction forces with the clean surfaces of MoS₂, which can be utilized for the fabrication of fluorescence-sensing assays for DNA and small biomolecules.