Issue 37, 2020, Issue in Progress

Ultrasonic-assisted enzymatic extraction of Sparassis crispa polysaccharides possessing protective ability against H2O2-induced oxidative damage in mouse hippocampal HT22 cells

Abstract

Sparassis crispa polysaccharides have recently attracted considerable attention due to their excellent bioactivities. However, their extraction procedure is often tedious, time-consuming, and environmentally unfriendly; it even causes damage to their structures and reduces their bioactivities, all of which hinder their further development to some extent. Therefore, the ultrasonic-assisted enzymatic extraction (UAEE) technology was optimized to extract polysaccharides from Sparassis crispa (SCP) by the response surface methodology. The yields, physicochemical properties, and antioxidant activities of SCPs obtained from UAEE and conventional hot water extraction (HWE) were evaluated. According to the optimal parameters, the yield of SCPs extracted by UAEE reached up to 14.63%, which increased by 68.54% compared with that obtained from the conventional hot water extraction (HWE) method. Additionally, the UAEE methods affected the contents of the polysaccharides, molecular weights, and the molar percentage of the constituent monosaccharides of SCPs. SEM analysis indicated that the microstructures of the two SCPs were notably different. Antioxidant assays showed that both SCPs possessed good antioxidant activities against DPPH, ABTSĖ™+, and hydroxyl radicals in vitro. Additionally, the SCPs extracted by UAEE attenuated the HT22 cell neurotoxicity induced by H2O2 by the means of ameliorating cell viability, reducing extracellular LDH release, and decreasing the levels of intracellular ROS. These results provide scientific basis for the further investigation of SCPs as potential neuroprotective agents.

Graphical abstract: Ultrasonic-assisted enzymatic extraction of Sparassis crispa polysaccharides possessing protective ability against H2O2-induced oxidative damage in mouse hippocampal HT22 cells

Supplementary files

Article information

Article type
Paper
Submitted
19 Feb 2020
Accepted
23 May 2020
First published
09 Jun 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 22164-22175

Ultrasonic-assisted enzymatic extraction of Sparassis crispa polysaccharides possessing protective ability against H2O2-induced oxidative damage in mouse hippocampal HT22 cells

W. Zhang, Y. Guo, Y. Cheng, W. Zhao, Y. Zheng and H. Qian, RSC Adv., 2020, 10, 22164 DOI: 10.1039/D0RA01581D

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