Visualizing antithrombin-binding 3-O-sulfated heparan sulfate motifs on cell surfaces

Mausam Kalita; Jie Shi Chua; Rio S. Boothello; April Joice; Orlando Antelope; Anindita Roy; Pon Velayutham Anandh Babu; Yukio Saijoh; Umesh R. Desai; Balagurunathan Kuberan

doi:10.1039/D0CC05893A

Visualizing antithrombin-binding 3-O-sulfated heparan sulfate motifs on cell surfaces†

Mausam Kalita,

^a Jie Shi Chua,^a Rio S. Boothello,

^bc April Joice,^a Orlando Antelope,^a Anindita Roy,^a Pon Velayutham Anandh Babu,

^d Yukio Saijoh,

^e Umesh R. Desai

*^bc and Balagurunathan Kuberan

*^a

Author affiliations

* Corresponding authors

^a Departments of Medicinal Chemistry and Bioengineering, University of Utah, Salt Lake City, UT 84112, USA
E-mail: kuby@pharm.utah.edu

^b Department of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University, Richmond, VA 23298, USA
E-mail: urdesai@vcu.edu

^c Institute for Structural Biology, Drug Discovery and Development, Virginia Commonwealth University, Richmond, VA 23219, USA

^d Department of Nutrition and Integrative Physiology, University of Utah, Salt Lake City, UT 84112, USA

^e Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84112, USA

Abstract

To map the cellular topography of the rare 3-O-sulfated structural motif of heparan sulfate (HS), we constructed quantum dot-based probes for antithrombin and FGF2, which reveal widely different distribution of the targeted HS motifs. The technology helps show that old and young aortic endothelia display widely different levels of the antithrombin-binding 3-O-sulfated HS motif.

Chemical Communications

Visualizing antithrombin-binding 3-O-sulfated heparan sulfate motifs on cell surfaces†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Visualizing antithrombin-binding 3-O-sulfated heparan sulfate motifs on cell surfaces

Social activity

Search articles by author

Spotlight

Advertisements