Issue 73, 2020

Reversible spatial and temporal control of lipid signaling

Abstract

Herein, we introduce versatile molecular tools that enable specific delivery and visualization of photoswitchable lipids at cellular membranes, namely at the plasma membrane and internal membranes. These molecules were prepared by tethering ortho-nitrobenzyl-based fluorescent cages with a signaling lipid bearing an azobenzene photoswitch. They permit two sequential photocontrolled reactions, which are uncaging of a lipid analogue and then its repeated activation and deactivation. We used these molecules to activate GPR40 receptor transiently expressed in HeLa cells and demonstrated downstream modulation of intracellular Ca2+ levels.

Graphical abstract: Reversible spatial and temporal control of lipid signaling

Supplementary files

Article information

Article type
Communication
Submitted
14 Jun 2020
Accepted
21 Aug 2020
First published
24 Aug 2020

Chem. Commun., 2020,56, 10646-10649

Reversible spatial and temporal control of lipid signaling

P. Gaur, M. Galkin, S. Hauke, R. Redkin, C. Barnes, V. V. Shvadchak and D. A. Yushchenko, Chem. Commun., 2020, 56, 10646 DOI: 10.1039/D0CC04146G

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