Issue 98, 2015
From the journal:

Chemical Communications

Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease†

Tongbo Wu,a   Xianjin Xiao,a   Feidan Gua  and  Meiping Zhao*a  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China
E-mail: mpzhao@pku.edu.cn
Fax: +86-10-62751708

Abstract

An abasic site modified fluorescent probe has been developed which enabled the rapid discrimination of stable single mismatched base pairs by lambda exonuclease with remarkably high discrimination factors (447 for T:G and 238 for A:G). This method allowed sensitive detection of single nucleotide variation at very low abundances (0.02–0.05% mutant-to-wild type).

Graphical abstract: Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease

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Article information

DOI
https://doi.org/10.1039/C5CC05749C
Article type
Communication
Submitted
11 Jul 2015
Accepted
06 Oct 2015
First published
06 Oct 2015

Chem. Commun., 2015,51, 17402-17405

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Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease

T. Wu, X. Xiao, F. Gu and M. Zhao, Chem. Commun., 2015, 51, 17402 DOI: 10.1039/C5CC05749C

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