A reusable and portable immunosensor using personal glucose meter as transducer

Xi Zhu; Hanye Zheng; Huifeng Xu; Ruolan Lin; Yejian Han; Guidi Yang; Zhenyu Lin; Longhua Guo; Bin Qiu; Guonan Chen

doi:10.1039/C4AY00490F

A reusable and portable immunosensor using personal glucose meter as transducer

Xi Zhu,^ab Hanye Zheng,^b Huifeng Xu,^bc Ruolan Lin,^a Yejian Han,^a Guidi Yang,^a Zhenyu Lin,*^b Longhua Guo,^b Bin Qiu^b and Guonan Chen*^b

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* Corresponding authors

^a College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

^b MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, China
E-mail: zylin@fzu.edu.cn, gnchen@fzu.edu.cn
Fax: +86-591-22866135
Tel: +86-591-22866135

^c Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, P.R. China

Abstract

In this work, a portable immunosensor was developed using a personal glucose meter (PGM) as the signal transducer. In this system, the anti-alpha-fetoprotein (anti-AFP) is immobilized on the screen-printed gold electrode (SPGE) surface. The presence of a target brought in the fixation of invertase–anti-AFP on anti-AFP modified SPGE; then, invertase catalyzed sucrose to glucose, which was detected using the PGM. The reading of the PGM exhibited a linear relationship with the AFP concentration. The detection limit is 0.18 ng mL⁻¹. After one test, this immunosensor can be reused at least 5 times using an easy treatment. Moreover, the assay results of the clinical serum samples are satisfactory.

Analytical Methods

A reusable and portable immunosensor using personal glucose meter as transducer

Abstract

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A reusable and portable immunosensor using personal glucose meter as transducer

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