Issue 37, 2012
From the journal:

Journal of Materials Chemistry

A biochemical logic approach to biomarker-activated drug release†

Vera Bocharova,ac   Oleksandr Zavalov,b   Kevin MacVittie,a   Mary A. Arugula,a   Nataliia V. Guz,b   Maxim E. Dokukin,b   Jan Halámek,a   Igor Sokolov,*b   Vladimir Privman*b  and  Evgeny Katz*a  

* Corresponding authors

a Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699, USA
E-mail: ekatz@clarkson.edu
Fax: +1 315-268-6610
Tel: +1 315-268-4421

b Department of Physics, Clarkson University, Potsdam, NY 13699, USA
E-mail: isokolov@clarkson.edu, privman@clarkson.edu
Fax: +1 315-268-7754
Tel: +1 315-268-2375

c Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6197, USA

Abstract

The present study aims at integrating drug-releasing materials with signal-processing biocomputing systems. Enzymes alanine transaminase (ALT) and aspartate transaminase (AST)—biomarkers for liver injury—were logically processed by a biocatalytic cascade realizing a Boolean AND gate. Citrate produced in the system was used to trigger a drug-mimicking release from alginate microspheres. In order to differentiate low vs. high concentration signals, the microspheres were coated with a protective shell composed of layer-by-layer adsorbed poly(L-lysine) and alginate. The alginate core of the microspheres was prepared from Fe3+-cross-linked alginate loaded with rhodamine 6G dye mimicking a drug. Dye release from the core occurred only when both biomarkers, ALT and AST, appeared at their high pathophysiological concentrations jointly indicative of liver injury. The signal-triggered response was studied at the level of a single microsphere, yielding information on the dye release kinetics.

Graphical abstract: A biochemical logic approach to biomarker-activated drug release

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2JM32966B
Article type
Paper
Submitted
10 May 2012
Accepted
23 Jul 2012
First published
25 Jul 2012

J. Mater. Chem., 2012,22, 19709-19717

Permissions

Request permissions

A biochemical logic approach to biomarker-activated drug release

V. Bocharova, O. Zavalov, K. MacVittie, M. A. Arugula, N. V. Guz, M. E. Dokukin, J. Halámek, I. Sokolov, V. Privman and E. Katz, J. Mater. Chem., 2012, 22, 19709 DOI: 10.1039/C2JM32966B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements