Issue 99, 2012
From the journal:

Chemical Communications

Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G†

Junya Chiba,*a   Takahide Kouno,*b   Shun Aoki,a   Hitoshi Sato,a   JingYing Zhang,b   Hiroshi Matsuob  and  Masahiko Inouyea  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan
E-mail: chiba@pha.u-toyama.ac.jp

b Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, 6-155 Jackson Hall, Minneapolis, MN 55455, USA
E-mail: konox005@umn.edu

Abstract

APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.

Graphical abstract: Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G

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Article information

DOI
https://doi.org/10.1039/C2CC36779C
Article type
Communication
Submitted
18 Sep 2012
Accepted
30 Oct 2012
First published
31 Oct 2012

Chem. Commun., 2012,48, 12115-12117

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Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G

J. Chiba, T. Kouno, S. Aoki, H. Sato, J. Zhang, H. Matsuo and M. Inouye, Chem. Commun., 2012, 48, 12115 DOI: 10.1039/C2CC36779C

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