Issue 4, 2013
From the journal:

Analyst

DNA barcoding via counterstaining with AT/GC sensitive ligands in injection-molded all-polymer nanochannel devices

Peter Friis Østergaard,a   Marco Matteucci,a   Walter Reisner†b  and  Rafael Taboryski†*a  

* Corresponding authors

a Department of Micro- and Nanotechnology, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark
E-mail: rata@nanotech.dtu.dk
Tel: +45 4525 8155

b Department of Physics, McGill University, Montreal, QC, Canada

Abstract

Nanochannel technology, coupled with a suitable DNA labeling chemistry, is a powerful approach for performing high-throughput single-molecule mapping of genomes. Yet so far nanochannel technology has remained inaccessible to the broader research community due to high fabrication cost and/or requirement of specialized facilities/skill-sets. In this article we show that nanochannel-based mapping can be performed in all polymer chips fabricated via injection molding: a fabrication process so inexpensive that the devices can be considered disposable. Fluorescent intensity variations can be obtained from molecules extended in the polymer nanochannels via chemical counterstaining against YOYO-1. In particular, we demonstrate that the counterstaining induced fluorescent intensity variations to a large degree appear to be proportional to the theoretically computed sequence-maps of both local AT and GC variation along DNA sequences.

Graphical abstract: DNA barcoding via counterstaining with AT/GC sensitive ligands in injection-molded all-polymer nanochannel devices

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Article information

DOI
https://doi.org/10.1039/C2AN36522G
Article type
Paper
Submitted
19 Oct 2012
Accepted
11 Dec 2012
First published
11 Dec 2012

Analyst, 2013,138, 1249-1255

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DNA barcoding via counterstaining with AT/GC sensitive ligands in injection-molded all-polymer nanochannel devices

P. F. Østergaard, M. Matteucci, W. Reisner and R. Taboryski, Analyst, 2013, 138, 1249 DOI: 10.1039/C2AN36522G

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