Issue 3, 2011
From the journal:

Analyst

G-Quadruplex-based DNAzyme for colorimetric detection ofcocaine: Using magnetic nanoparticles as the separation and amplification element†

Yan Du,ab   Bingling Li,ab   Shaojun Guo,ab   Zhixue Zhou,ab   Ming Zhou,ab   Erkang Wang*a  and  Shaojun Dong*a  

* Corresponding authors

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, P. R. China
E-mail: dongsj@ciac.jl.cn, ekwang@ciac.jl.cn
Fax: (+86) 431-85689711
Tel: (+86) 431-85262101

b Graduate School of the Chinese Academy of Sciences, Beijing, P. R. China

Abstract

The appearance of the aptamer provides good recognition elements for small molecules, especially for drugs. In this work, by combining the advantages of magnetic nanoparticles (MNPs) with colorimetric drug detection using hemin-G-quadruplex complex as the sensing element, we report a simple and sensitive DNAzyme-based colorimetric sensor for cocaine detection in a 3,3,5,5-tetramethylbenzidine sulfate (TMB)–H2O2 reaction system. The whole experimental processes are simplified. Cocaine aptamer fragments, SH–C2, are covalently labeled onto the amine-functionalized MNPs. When the target cocaine and another cocaine aptamer fragments (C1) grafted with G-riched strand AG4 (i.e. C1–AG4) are present simultaneously, the C2 layer on MNPs hybridizes partly with C1–AG4 to bind the cocaine. The C1–AG4 can be combinded with hemin to form DNAzyme which can effectively catalyze the H2O2-mediated oxidation of TMB, giving rise to a change in solution color. Importantly, using MNPs as the separation and amplification elements could effectively reduce the background signal and the interference from the real samples. A linear response from 0.1 μM to 20 μM is obtained for cocaine and a detection limit of 50 nM is achieved, which provides high sensitivity and selectivity to detect cocaine.

Graphical abstract: G-Quadruplex-based DNAzyme for colorimetric detection of cocaine: Using magnetic nanoparticles as the separation and amplification element

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C0AN00557F
Article type
Paper
Submitted
21 Jul 2010
Accepted
28 Oct 2010
First published
16 Nov 2010

Analyst, 2011,136, 493-497

Permissions

Request permissions

G-Quadruplex-based DNAzyme for colorimetric detection of cocaine: Using magnetic nanoparticles as the separation and amplification element

Y. Du, B. Li, S. Guo, Z. Zhou, M. Zhou, E. Wang and S. Dong, Analyst, 2011, 136, 493 DOI: 10.1039/C0AN00557F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements