Issue 36, 2009
From the journal:

Journal of Materials Chemistry

Synthesis, characterization and protein binding properties of supported dendrons†

Olga Iliashevsky,a   Liron Amir,ab   Robert Glaser,a   Robert S. Marksb  and  N. Gabriel Lemcoff*a  

* Corresponding authors

a Department of Chemistry, Ben-Gurion University of the Negev, P.O.Box 653, Beer-Sheva, Israel
E-mail: lemcoff@bgu.ac.il
Fax: (+97)-8-6461740
Tel: (+97)-8-6461196

b Department of Biochemical Engineering, Ben-Gurion University of the Negev, P.O.Box 653, Beer-Sheva, Israel

Abstract

Novel benzylether type aldehyde and acetal terminated dendrons were synthesized and attached to a silica gel support; a linear spacer was also introduced as a control material. The supported dendritic compounds were mainly characterized by solid state 13C CPMAS NMR, elemental analysis and X-ray photoelectron spectroscopy (XPS) and the presence of free aldehydes was determined by the purpald test. Bovine serum albumin (BSA) protein was coupled to the dendronized support by imine bond formation, followed by irreversible reduction of the carbon–nitrogen double bond. A significant positive dendritic effect was observed on the antibody binding capacity of immobilised BSA as measured by fluorescence immunoassay (FIA).

Graphical abstract: Synthesis, characterization and protein binding properties of supported dendrons

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Article information

DOI
https://doi.org/10.1039/B908014G
Article type
Paper
Submitted
22 Apr 2009
Accepted
11 Jun 2009
First published
10 Jul 2009

J. Mater. Chem., 2009,19, 6616-6622

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Synthesis, characterization and protein binding properties of supported dendrons

O. Iliashevsky, L. Amir, R. Glaser, R. S. Marks and N. G. Lemcoff, J. Mater. Chem., 2009, 19, 6616 DOI: 10.1039/B908014G

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