Issue 7, 2007
From the journal:

Analyst

Differentiation of individual human mesenchymalstem cells probed by FTIR microscopic imaging

Christoph Krafft,†*a   Reiner Salzer,a   Sebastian Seitz,b   Christina Ernb  and  Matthias Schiekerb  

* Corresponding authors

a Institute for Analytical Chemistry, Dresden University of Technology, D-01062 Dresden, Germany
E-mail: christoph.krafft@tu-dresden.de

b Experimental Surgery and Regenerative Medicine, Department of Surgery, Ludwigs-Maximilian-University Munich, Nussbaumstr. 20, D-80336 Muenchen, Germany

Abstract

Objective of this study is the novel application of Fourier transform infrared (FTIR) microscopic imaging to identify the differentiation state of individual human mesenchymalstem cells with or without osteogenic stimulation. IR spectra of several hundred single cells with lateral resolution of 5–10 µm were recorded using a FTIR imaging spectrometer coupled to a microscope with a focal plane array detector. A classification model based on linear discriminant analysis was trained to distinguish four cell types by their IR spectroscopic fingerprint. Without stimulation two cell types dominated, showing low or high levels of glycogen accumulation at the cell periphery. After stimulation, the protein composition in the cells changed and some cells started expressing calcium phosphate salts such as octacalciumphosphate, a precursor of the bone constituent hydroxyapatite. Few cells were identified which remained in their non-stimulated state. This study demonstrated for the first time that FTIR microscopic imaging can probe stem cell differentiation at the single cell level rapidly, non-destructively and with minimal preparation.

Graphical abstract: Differentiation of individual human mesenchymalstem cells probed by FTIR microscopic imaging

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Article information

DOI
https://doi.org/10.1039/B700368D
Article type
Paper
Submitted
09 Jan 2007
Accepted
14 May 2007
First published
24 May 2007

Analyst, 2007,132, 647-653

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Differentiation of individual human mesenchymalstem cells probed by FTIR microscopic imaging

C. Krafft, R. Salzer, S. Seitz, C. Ern and M. Schieker, Analyst, 2007, 132, 647 DOI: 10.1039/B700368D

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