Issue 9, 2006
From the journal:

Journal of Environmental Monitoring

Earthworm cytochrome P450 determination and application as a biomarker for diagnosing PAH exposure

W. Zhang,ab   Y. F. Song,*ac   P. Gong,d   T. H. Sun,ac   Q. X. Zhouac  and  M. Liuab  

* Corresponding authors

a Key Laboratory of Terrestrial Ecological Processes, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P. R. China
E-mail: zhangweilm04@163.com
Fax: 86 24 8397 0436
Tel: 86 24 8397 0426

b Graduate School, Chinese Academy of Sciences, Beijing, P. R. China

c Key Laboratory of Environmental Engineering, Shenyang University, Shenyang, P. R. China
E-mail: Songyufang@iae.ac.cn

d SpecPro Inc., 3909 Halls Ferry Road, Vicksburg, Mississippi, USA
E-mail: ping.gong@us.army.mil

Abstract

We developed a new microsome purification method and used the Omura and Sato method to measure the total content of cytochrome (Cyt) P450 in earthworm (Eisenia fetida) microsomes. In method development, two different pretreatments, i.e. solubilization or manual separation were used to purify worm microsomes. Solubilization was more effective than manual separation and difference spectra showed a peak at 450 ± 1 nm in microsomes received solubilization pretreatment. We conducted a 48 h contact test by exposing worms on pyrene (Py) and benzo[a]pyrene (BaP) spiked filter paper. A dose-response relationship was established between total P450 content and the concentration of Py or BaP ranging from 10−6 mg mL−1 to 10−2 mg mL−1. Results show that total Cyt P450 content in earthworms is a promising biomarker for diagnosing PAHs exposure at sublethal dose ranges.

Graphical abstract: Earthworm cytochrome P450 determination and application as a biomarker for diagnosing PAH exposure

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Article information

DOI
https://doi.org/10.1039/B605450A
Article type
Paper
Submitted
18 Apr 2006
Accepted
10 Jul 2006
First published
17 Jul 2006

J. Environ. Monit., 2006,8, 963-967

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Earthworm cytochrome P450 determination and application as a biomarker for diagnosing PAH exposure

W. Zhang, Y. F. Song, P. Gong, T. H. Sun, Q. X. Zhou and M. Liu, J. Environ. Monit., 2006, 8, 963 DOI: 10.1039/B605450A

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