Volume 122, 2003
From the journal:

Faraday Discussions

Structural dynamics of the receptor-binding domain of colicin E9

Ruth Boetzel,a   Emily S. Collins,a   Nigel J. Clayden,a   Colin Kleanthous,b   Richard Jamesc  and  Geoffrey R. Moore*a  

* Corresponding authors

a School of Chemical Sciences, University of East Anglia, Norwich, UK
E-mail: g.moore@uea.ac.uk

b School of Biological Sciences, University of East Anglia, Norwich, UK

c Division of Microbiology and Infectious Diseases, School of Laboratory Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, UK

Abstract

Colicin E9 is a 61 kDa antibacterial protein secreted by E. coli. In order for it to enter the cytoplasm of susceptible bacteria and kill them by hydrolysing their DNA, the colicin must first interact with an outer membrane receptor on the target cell, BtuB, and a translocation pathway involving Tol proteins. The receptor binding, translocation and DNase functions of colicin E9 are housed in discrete structural domains, which have been independently expressed and characterized. The minimal receptor-binding domain is a 76 amino acid protein (min-R). X-ray structure determination of a related colicin shows its receptor-binding domain to have a helical hairpin structure (S. Soelaiman, K. Jakes, N. Wu, C. Li and M. Shoham, Molecular Cell, 2001, 8, 1053). Our solution NMR studies of min-R have confirmed it has a helical hairpin structure, and shown it has multiple slowly interchanging conformers and a flexible inter-helix loop. A plausible interpretation of these data is that in solution the helical hairpin can adopt a variety of structures differing in the spatial relationship of the two helices. A possible biological role for this involves the hairpin opening during translocation into bacteria.

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Article information

DOI
https://doi.org/10.1039/B201127A
Article type
Paper
Submitted
30 Jan 2002
Accepted
04 Apr 2002
First published
07 Oct 2002

Faraday Discuss., 2003,122, 145-162

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Structural dynamics of the receptor-binding domain of colicin E9

R. Boetzel, E. S. Collins, N. J. Clayden, C. Kleanthous, R. James and G. R. Moore, Faraday Discuss., 2003, 122, 145 DOI: 10.1039/B201127A

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