Resolution equations for column chromatography
Abstract
The concept of resolution in column chromatography is briefly reviewed. Two new resolution equations are compared with others previously introduced and are shown to be considerably more accurate than the most commonly employed resolution equations. The new equations are identical in form with the most commonly employed equations, a distinct advantage over previously introduced alternatives. For situations in which the plate counts of the peaks of interest differ by more than 20%, an accurate empirical resolution equation is proposed.