Abstract
WHEN two populations of allogeneic lymphoid cells are cultured together, cellular interactions lead to blast transformation and proliferation of a portion of the cultured cells. In mice, this mixed leukocyte culture reaction (MLR) is under the independent control of two genetic systems: the multigenic major (H-2) histocompatibility system (MHS)1,2 and the unigenic (non-H-2) M locus3. The nature and cellular distribution of the gene products which mediate recognition of one cell (the stimulator cell) by another cell (the responder cell) are incompletely defined. The ‘recognising’ structure is likely to be a specific receptor present on thymus-derived (T) lymphocytes, since T cells are generally required as responder cells in MLR3–6, Recently it has been possible to distinguish, from these recognition sites, gene products which are expressed on the stimulator cells7 and which have been termed lymphocyte-activating determinants (LADs)3. Cell fractionation studies revealed that LADs of the MHS are expressed on both B and T lymphocytes8,9, whereas LADs of the M locus are preferentially if not exclusively expressed on B lymphocytes10. In this study we present evidence that LADs of the M locus as well as LADs of the MHS are also expressed on macrophages. These genetic determinants are shown to be stable in long term cultures and to induce specific one-way reactions in mixed lymphocyte–macrophage cultures.
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SCHIRRMACHER, V., PEÑA-MARTINEZ, J. & FESTENSTEIN, H. Specific lymphocyte-activating determinants expressed on mouse macrophages. Nature 255, 155–156 (1975). https://doi.org/10.1038/255155a0
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DOI: https://doi.org/10.1038/255155a0
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