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In vitro maturation in synthetic oviductal fluid increases gene expression associated with quality and lipid metabolism in bovine oocytes

Published online by Cambridge University Press:  13 November 2023

Adriano Felipe Mendes ,
Raquel Zaneti Puelker ,
Lilian Francisco Arantes de Souza ,
Andréa Renesto Coimbra Jacintho ,
Priscila Helena dos Santos ,
Ines Cristina Giometti ,
Sheila Merlo Firetti ,
Anthony César de Souza Castilho ,
Marilice Zundt  and
Claudia Maria Bertan Membrive
...Show all authors
Adriano Felipe Mendes
Affiliation:
University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil
Raquel Zaneti Puelker
Affiliation:
São Paulo State University (UNESP), Botucatu, São Paulo, Brazil
Lilian Francisco Arantes de Souza
Affiliation:
University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil
Andréa Renesto Coimbra Jacintho
Affiliation:
University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil
Priscila Helena dos Santos
Affiliation:
São Paulo State University (UNESP), Botucatu, São Paulo, Brazil
Ines Cristina Giometti
Affiliation:
University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil
Sheila Merlo Firetti
Affiliation:
University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil
Anthony César de Souza Castilho
Affiliation:
University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil São Paulo State University (UNESP), Botucatu, São Paulo, Brazil
Marilice Zundt
Affiliation:
University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil
Claudia Maria Bertan Membrive
Affiliation:
São Paulo State University (UNESP), Dracena, São Paulo, Brazil
Caliê Castilho*
Affiliation:
University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil
*
Corresponding author: Caliê Castilho; Email: calie@unoeste.br
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Summary

Traditionally, in vitro oocyte and embryo culture progresses through a series of varying culture medium. To investigate simplifying the in vitro production of bovine cumulus–oocyte complexes (COCs), this study used synthetic oviductal fluid (SOF) supplemented with conjugated linoleic acid (CLA). Special interest was placed on gene expression linked to lipid metabolism and oocyte maturation. COCs were matured in different media: Medium 199 (M199 group), M199 with 100 μM CLA (M199 + CLA group), SOF (SOF group), and SOF with 100 μM CLA (SOF + CLA group). COCs matured with SOF showed a higher relative abundance of mRNA of quality indicators gremlin 1 (GREM1) and prostaglandin-endoperoxide synthase 2 (PTGS2) in oocytes, and GREM1 in cumulus cells compared with in the M199 group. SOF medium COCs had a higher relative abundance of fatty acid desaturase 2 (FADS2) compared with the M199 group, which is essential for lipid metabolism in oocytes. Furthermore, the abundance of stearoyl-coenzyme A desaturase 1 (SCD1) in oocytes matured with SOF was not influenced by the addition of CLA, whereas the relative abundance of SCD1 was reduced in M199 medium with CLA. We concluded that maturation in SOF medium results in a greater abundance of genes linked to quality and lipidic metabolism in oocytes, regardless of the addition of CLA.

Keywords

Conjugated linoleic acidCumulus–oocyte complexesIVFLipid metabolismSynthetic oviductal fluid
Type
Research Article
Information
Zygote , Volume 31 , Issue 6 , December 2023 , pp. 582 - 587
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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