Hostname: page-component-848d4c4894-pftt2 Total loading time: 0 Render date: 2024-05-01T15:13:46.826Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of the human sperm nucleus: ambiguity and risk of confusion with chromomycin staining

Published online by Cambridge University Press:  20 January 2021

Yves Ménézo Open the ORCID record for Yves Ménézo [Opens in a new window]
Yves Ménézo*
Affiliation:
Laboratoire Clément, Paris, France
*
Author for correspondence: Yves Ménézo. Laboratoire Clément, 75016Paris, France. E-mail: yves.menezo@gmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

An abstract is not available for this content so a preview has been provided. Please use the Get access link above for information on how to access this content.
Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
Commentary
Information
Zygote , Volume 29 , Issue 4 , August 2021 , pp. 257 - 259
Copyright
© The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aitken, RJ, De Iuliis, GN, Finnie, JM, Hedges, A and McLachlan, R (2010). Hum Reprod 25, 2415–226.CrossRefGoogle Scholar
Badouard, C, Ménézo, Y, Panteix, G, Ravanat, JL, Douki, T, Cadet, J and Favier, A (2008). Determination of new types of DNA lesions in human sperm. Zygote 16, 9–13.CrossRefGoogle ScholarPubMed
Belloc, S, Benkhalifa, M, Junca, AM, Dumont, M, Bacrie, PC and Ménézo, Y (2009). Paternal age and sperm DNA decay: discrepancy between chromomycin and aniline blue staining. Reprod Biomed Online. 19, 264–9.CrossRefGoogle ScholarPubMed
Booze, M, Brannian, J, Von Wald, T, Hansen, K and Evenson, D (2019). High DNA stability in the SCSA is associated with poor embryo development and live birth outcome. American Association of Bioanalysts Conference. May 16–18. New Orleans, LA. [abstract].Google Scholar
Buck Louis, GM, Sundaram, R, Schisterman, EF, Sweeney, A, Lynch, CD, Kim, S, Maisog, JM, Gore-Langton, R, Eisenberg, ML and Chen, Z (2014). Semen quality and time to pregnancy: the Longitudinal Investigation of Fertility and the Environment Study. Fertil Steril 101, 453–462.CrossRefGoogle ScholarPubMed
Dattilo, M, D’Amato, G, Caroppo, E and Ménézo, Y (2016). Improvement of gamete quality by stimulating and feeding the endogenous antioxidant system: mechanisms, clinical results, insights on gene-environment interactions and the role of diet. J Assist Reprod Genet 33, 1633.CrossRefGoogle ScholarPubMed
Deenadayal Mettler, A, Govindarajan, M, Srinivas, S, Mithraprabhu, S, Evenson, D and Mahendran, T (2019). Male age is associated with sperm DNA/chromatin integrity. Aging Male 9, 1–8.Google Scholar
De Iuliis, GN, Thomson, LK, Mitchell, LA, Finnie, JM, Koppers, AJ, Hedges, A, Nixon, B and Aitken, RJ (2009). DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2-deoxyguanosine, a marker of oxidative stress. Biol Reprod 81, 517–24.CrossRefGoogle ScholarPubMed
Eid, LN, Lorton, SP and Parrish, JJ (1994). Paternal influence on S-phase in the first cell cycle of the bovine embryo. Biol Reprod 51, 1232–7.CrossRefGoogle ScholarPubMed
Evenson, DP, Jost, LK, Marshall, D, Zinaman, MJ, Clegg, E, Purvis, K, de Angelis, P and Claussen, OP (1999). Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod 14, 1039–49.CrossRefGoogle ScholarPubMed
Evenson, DP, Djira, G, Kasperson, K and Christianson, J (2020). Relationships between the age of 25,445 men attending infertility clinics and sperm chromatin structure assay (SCSA®) defined sperm DNA and chromatin integrity. Fertil Steril 114, 311–320.CrossRefGoogle ScholarPubMed
Gallo, A, Ménézo, Y, Dale, B, Coppola, G, Dattilo, M, Tosti, E and Boni, R (2018). Metabolic enhancers supporting 1-carbon cycle affect sperm functionality: an in vitro comparative study. Sci Rep 8, 11769.CrossRefGoogle Scholar
Hamidi, J, Frainais, C, Amar, E, Bailly, E, Clément, P and Ménézo, Y (2015). A double-blinded comparison of in situ TUNEL and aniline blue versus flow cytometry acridine orange for the determination of sperm DNA fragmentation and nucleus decondensation state index. Zygote 234, 556–62.CrossRefGoogle Scholar
Jacquesson-Fournols, L, Alvarez, S, Cohen, M, Clément, P and Ménézo, Y (2019). A paternal effect of MTHFR SNPs on gametes and embryos should not be overlooked: case reports. J Assist Reprod Genet 36, 1351–3.CrossRefGoogle Scholar
Jerre, E, Bungum, M, Evenson, D and Giwercman, A (2019). Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection. Fertil Steril 112, 46–53.CrossRefGoogle ScholarPubMed
Junca, A, Gonzalez Marti, B, Tosti, E, Cohen, M, De la Fontaine, D, Benkhalifa, M and Ménézo, Y (2012). Sperm nucleus decondensation, hyaluronic acid (HA) binding and oocyte activation capacity: different markers of sperm immaturity? Case reports. J Assist Reprod Genet 29, 353–5.CrossRefGoogle ScholarPubMed
Kutchy, NA, Menezes, ESB, Ugur, MR, Ul Husna, A, ElDebaky, H, Evans, HC, Beaty, E, Santos, FC, Tan, W, Wills, RW, Topper, E, Kaya, A, Moura, AA and Memili, R (2019). Sperm cellular and nuclear dynamics associated with bull fertility. Anim Reprod Sci 211, 106203.CrossRefGoogle ScholarPubMed
Lopes, S, Jurisicova, A and Casper, RF (1998). Gamete-Specific DNA fragmentation in unfertilized human oocytes after intracytoplasmic sperm injection. Hum Reprod 13, 703–8.CrossRefGoogle ScholarPubMed
Manicardi, GC, Bianchi, PG, Pantano, S, Azzoni, P, Bizzaro, D, Bianchi, U and Sakkas, D (1995). Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility. Biol Reprod 52, 864–7.CrossRefGoogle ScholarPubMed
Ménézo, Y, Russo, GL, Tosti, E, El Mouatassim, S and Benkhalifa, MJ (2007). Expression profile of genes coding for DNA repair in human oocytes using pangenomic microarrays, with a special focus on ROS linked decays. J. Assist Reprod Genet 24, 513–20.CrossRefGoogle ScholarPubMed
Ménézo, Y, Dale, B and Cohen, MD (2010). DNA damage and repair in human oocytes and embryos: a review. Zygote 18, 357–65.CrossRefGoogle ScholarPubMed
Ménézo, Y, Entezami, F, Lichtblau, I, Belloc, S, Cohen, M and Dale, B (2014a). Oxidative stress and fertility: incorrect assumptions and ineffective solutions? Zygote 22, 80–90.CrossRefGoogle ScholarPubMed
Ménézo, Y, Evenson, D, Cohen, M and Dale, B (2014b). Effect of antioxidants on sperm genetic damage. Adv Exp Med Biol 791, 173–89.CrossRefGoogle ScholarPubMed
Mohammadi, Z, Tavalaee, M, Gharagozloo, P, Drevet, JR and Nasr-Esfahani, MH (2020). Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity? Basic Clin Androl 30, 12.CrossRefGoogle ScholarPubMed
Paoli, D, Pecora, G, Pallotti, F, Faja, F, Pelloni, M, Lenzi, A and Lombardo, F (2019). Cytological and molecular aspects of the ageing sperm. Hum Reprod 34, 218–27.CrossRefGoogle ScholarPubMed
Park, JS, Jeong, YS, Shin, ST, Lee, KK and Kang, YK (2007). Dynamic DNA methylation reprogramming: active demethylation and immediate remethylation in the male pronucleus of bovine zygotes. Dev Dyn 236, 2523–33.CrossRefGoogle ScholarPubMed
Rahman, MB, Schellander, K, Luceño, NL and Van Soom, A (2018). Heat stress responses in spermatozoa: mechanisms and consequences for cattle fertility. Theriogenology 113, 102–12.CrossRefGoogle ScholarPubMed
Ward, F, Rizos, D, Corridan, D, Quinn, K, Boland, M and Lonergan, P (2001). Paternal influence on the time of first embryonic cleavage post insemination and the implications for subsequent bovine embryo development in vitro and fertility in vivo . Mol Reprod Dev 60, 47–55.CrossRefGoogle ScholarPubMed
Ward, WS (2010). Function of sperm chromatin structural elements in fertilization and development. Mol Hum Reprod 16, 30–6.CrossRefGoogle ScholarPubMed
Zini, A, Phillips, S, Courchesne, A, Boman, JM, Baazeem, A, Bissonnette, F, Kadoch, IJ and San Gabriel, M (2009). Sperm head morphology is related to high deoxyribonucleic acid stainability assessed by sperm chromatin structure assay. Fertil Steril 91, 2495–500.CrossRefGoogle ScholarPubMed