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Quality of Pinzgau bull spermatozoa following different periods of cryostorage

Published online by Cambridge University Press:  09 March 2017

P. Chrenek ,
E. Spaleková ,
L. Olexikova ,
A. Makarevich  and
E. Kubovicova
P. Chrenek*
Affiliation:
National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Slovak Republic. Slovak University of Agriculture in Nitra, Slovak Republic.
E. Spaleková
Affiliation:
09National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Slovak Republic.
L. Olexikova
Affiliation:
09National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Slovak Republic.
A. Makarevich
Affiliation:
09National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Slovak Republic.
E. Kubovicova
Affiliation:
09National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Slovak Republic.
*
All correspondence to: Peter Chrenek. National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Slovak Republic. E-mail: chrenekp@vuzv.sk
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Summary

The aim of this work was to examine the influence of cryostorage duration of Pinzgau bull's insemination doses (IDs) on some sperm traits. The IDs were frozen by a slow freezing method and stored in liquid nitrogen for different periods: less than 8 years (group 1), 8–13 years (group 2) and 14–18 years (group 3). Motility (CASA), pathological sperm rate (Giemsa staining), apoptotic (Yo-Pro-1-positive) and necrotic (propidium iodide-positive) cell occurrence and fertilizing ability (penetration/fertilization test) of spermatozoa were evaluated post-thaw. The average post-thaw sperm motility in all examined groups was over 40%. No significant influence of storage length either on the sperm total motility or progressive movement was revealed. In each tested group the average rate of malformed spermatozoa did not exceed 20%. No effect of cryostorage length on the occurrence of apoptotic or necrotic sperm was noted. Similarly, penetrating/fertilizing ability of sperm did not differ among the groups, excepting differences in the rate of pronuclei (PN) formation. In group 1, 72.9% of eggs showed two visible PN following 20 h incubation with sperm, whilst in groups 2 and 3 only 67 and 54.5% of zygotes, respectively, had both PN at this time. These results revealed no influence of storage time on the bull spermatozoa in all parameters excepting the rate of PN formation. As high inter-male variability was observed in the susceptibility of bull sperm to cryostorage, individual differences should be taken into account when semen from individual bulls is to be stored for a long time.

Keywords

BullCryopreservationMotilityPinzgauSpermViability
Type
Research Article
Information
Zygote , Volume 25 , Issue 2 , April 2017 , pp. 215 - 221
Copyright
Copyright © Cambridge University Press 2017 

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