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Observations on the growth of Eimeria tenella in cultured cells from the parasitized chorioallantoic membranes of the developing chick embryo

Published online by Cambridge University Press:  06 April 2009

P. L. Long
P. L. Long*
Affiliation:
Houghton Poultry Research Station, Houghton, Huntingdon and Brunel University, Uxbridge, Middlesex
*
*This work was carried out during sabbatical leave at the Department of Avian Diseases, N.Y. State Veterinary College, Cornell University, Ithaca, N.Y., U.S.A.
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Extract

Eimeria tenella infections were established in the chorioallantoic membranes CAM) of developing chick embryos. At different times after infection the CAM ells were cultured in vitro in modified 199 medium. Different types of schizont were grown, some similar to those occurring in infections of the usual in vivo site and others markedly different. In schizogony of one type the merozoites appeared to develop by growing out from the periphery of cellular masses; a process similar to that described by Hammond et al. (1966) for E. bovis.

Gametocytes and infective oocysts were also grown by the tissue culture of CAM cells but only in cells infected 4–6 days previously in embryo; CAM cells infected for only 3 days before culture supported the growth of large numbers of schizonts of different types.

The majority of the stages grown developed within densely populated areas of epithelial-like cells. A temperature of 41 °C was found to be necessary for the growth of the parasite. The nutritional requirements for the growth of E. tenella are fairly well provided by the medium used. The relatively simple procedure described should be of value for the observation of E. tenella at various stages of its growth and provide a means of in vitro testing of antiparasitic substances.

I wish to thank Dr P. P. Levine for his interest and encouragement during the course of the work and Mr D. L'Amoreaux for technical assistance.

Type
Research Article
Information
Parasitology , Volume 59 , Issue 4 , November 1969 , pp. 757 - 765
Copyright
Copyright © Cambridge University Press 1969

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References

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