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Histochemical observations on carbohydrates, lipids and enzymes in digenean parasites and host tissues of Bithynia tentaculata

Published online by Cambridge University Press:  06 April 2009

Trevor A. J. Reader
Trevor A. J. Reader
Affiliation:
Department of Biological Sciences, Portsmouth Polytechnic, Hay Street, Portsmouth
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Extract

Active digenean larvae in Bithynia tentaculata were found to utilize both host carbohydrates and lipids, and these were incorporated into the bodies of developing cercariae. Although rediae obtain most of their nutrients by direct ingestion, a decrease in the glycogen and neutral fat content of intact digestive gland cells of snails infected with rediae suggests that these larvae may also absorb nutrients through their body walls, like sporocysts. In sporocyst infections, host glycogen is believed to be hydrolysed to glucose and other monosaccharides by the host's own enzymes, possibly phosphatases, which have been stimulated to greater activity by the parasites. These simple sugars, due to their small size, can then be absorbed through the sporocyst body wall. Similarly, host fats are probably degraded to fatty acids, possibly by a parasite-secreted lipase, prior to absorption by the parasites. Encysted metacercariae do not appear to feed, but they do have severe pressure effects which disrupt some of the digestive gland tubules. Such tubules show an increase in their neutral fat content and a decrease in phosphatase activity. Aminopeptidase enzymes secreted by active digenean larvae may be responsible for some lysis of host cells, and may also be involved in the breakdown of lymph proteins to amino acids.

I am indebted to Dr F. R. Stranack for her supervision and guidance throughout this investigation, and to Dr G. Charles and Dr T. Jenkins for their continued help and advice. This study was carried out during the tenure of a research assistantship awarded by the governors of Portsmouth Polytechnic.

Type
Research Article
Information
Parasitology , Volume 63 , Issue 1 , August 1971 , pp. 125 - 136
Copyright
Copyright © Cambridge University Press 1971

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References

REFERENCES

Axmann, M. C. (1947). Morphological studies on glycogen deposition in Schistosomes and other flukes. Journal of Morphology 80, 321–34.Google Scholar
Cain, A. J. (1947). An examination of Baker's acid haematein test for phospholipines. Quarterly Journal of Microscopical Science 88, 467–78.Google Scholar
Cheng, T. C. (1962). The effects of parasitism by the larvae of Echinoparyphium Dietz (Trematoda: Echinostomatidae) on the structure of and glycogen deposition in the hepatopancreas of Helisoma trivolvis (Say). American Zoologist 2, 513.Google Scholar
Cheng, T. C. (1963 a). Histological and histochemical studies on the effects of parasitism of Musculium partumeium (Say) by the larvae of Gorgodera amplicava. Loose. Proceedings of the Helminthological Society, Washington 30, 101–7.Google Scholar
Cheng, T. C. (1963 b). The effects of Echinoparyphium larvae on the structure of an glycogen deposition in the hepatopancreas of Helisoma trivolvis and glycogenesis in the parasite larvae. Malacologia 1, 291–303.Google Scholar
Cheng, T. C. (1963 c). Biochemical requirements of larval trematodes. Annals of the New York Academy of Sciences 113, 289–321.CrossRefGoogle ScholarPubMed
Cheng, T. C. (1964). Studies on phosphatase systems in hepatopancreatic cells of the molluscan host of Echinoparyphium sp. and in the rediae and cercariae of this trematode. Parasitology 54, 73–9.Google Scholar
Cheng, T. C. (1965). Histochemical observations on changes in the lipid composition of the American oyster Crassostrea virginica (Gmelin), parasitised by the trematode Bucephalus sp. Journal of Invertebrate Pathology 7, 398–407.CrossRefGoogle Scholar
Cheng, T. C. & Burton, R. W. (1966). Relationships between Bucephalus sp. and Crassostrea virginica: a histochemical study of some carbohydrates and carbohydrate complexes occurring in the host and parasite. Parasitology 56, 111–22.Google Scholar
Cheng, T. C. & Snyder, R. W. Jr. (1962 a). Studies on host-parasite relationships between larval trematodes and their hosts. I, A review. II, The utilization of the host's glycogen by the intramolluscan larvae of Glypthelmins pennsylvaniensis Cheng, and associated phenomena. Transactions of the American Microscopical Society 81, 209–28.Google Scholar
Cheng, T. C. & Snyder, R. W. Jr. (1962 b). Studies on the host—parasite relationships between larval trematodes and their hosts. III. Certain aspects of lipid metabolism in Helisoma trivolvis infected with rediae of Glypthelmins pennsylvaniensis and related phenomena. Transactions of the American Microscopical Society 81, 327–31.Google Scholar
Cheng, T. C. & Snyder, R. W. Jr. (1963). Studies on host—parasite relationships between larval trematodes and their hosts. IV. A histochemical determination of glucose and its role in the metabolism of molluscan and parasite. Transactions of the American Microscopical Society 82, 343–6.Google Scholar
Cheng, T. C. & Yee, H. W. F. (1968). Histochemical demonstration of aminopeptidase activity associated with the intramolluscan stages of Philopthalmus gralli Mathis and Leger. Parasitology 58, 473–80.CrossRefGoogle Scholar
Chiffelle, T. L. & Putt, F. A. (1951). Propylene and ethylene glycol as solvents for Sudan IV and Sudan black B. Stain technology 26, 51–6.CrossRefGoogle ScholarPubMed
Dubois, G. (1927). Les cercaires de Ia région de Neuchâtel. Society Neuchâteloise des Sciences Naturelle 53, 1–77.Google Scholar
Ginetsinskaya, T. A. (1960). The relationship between the distribution of glycogen in the bodies of different cercariae and their biological peculiarities. Doklady Akademii Nauk SSSR 135, 1012–15. (In Russian.)Google Scholar
Ginetsinskaya, T. A. (1961). Dynamics of fat deposition in the course of trematode life cycles. Doklady Akademii Nauk SSSR 139, 1016–19. (In Russian.)Google Scholar
Gomori, G. (1952). Microscopic Histochemistry. Chicago: University of Chicago Press.Google Scholar
James, B. L. (1965). The effects of parasitism by larval Digenea on the digestive gland of the intertidal prosobranch, Littorina saxatilis (Olivi) subsp. tenebrosa (Montagu). Parasitology 55, 93–115.Google Scholar
James, B. L. & Bowers, E. A. (1967). Histochemical observations on the occurrence of carbohydrates, lipids and enzymes in the daughter sporocyst of Cercaria bucephaloppsis haimaena Lacaze-Duthiers, 1854 (Digenea: Bucephalidae). Parasitology 57, 79–86.CrossRefGoogle Scholar
Lillie, R. D. (1944). Various oil soluble dyes as fat stains in the supersaturated isopropanol technic. Stain Technology 19, 55–8.Google Scholar
Looss, A. (1900). Recherches sur la faune parasitaire de l'Egypte: I. Mémorandum d'Institut égyptien 3, 1–252.Google Scholar
LÜhe, M. (1909). Parasitische Plattwurmer. 1. Trematoden. Su Bwasserfauna Deutschlands 17, 1–217.Google Scholar
Lutta, A. D. (1939). The dynamics of the glycogen and fat in parasitic worms. Uchen Zapiski Leningrad Gosudarstv. University, Series 11, 129–71. (In Russian.)Google Scholar
Nachlas, M. M., Crawford, D. T. & Seligman, A. M. (1957). The histochemical demonstration of leucine aminopeptidase. Journal of Histochemistry and Cytochemistry 5, 264–78.CrossRefGoogle ScholarPubMed
Negus, M. R. S. (1968). The nutrition of sporocysts of the trematode Cercaria doricha Rothschild, 1935 in the molluscan host Turritella communis Risso. Parasitology 58, 355–66.CrossRefGoogle Scholar
Odhner, T. (1910). Nordafricanische Trematoden grosstenteils vom weissen Nil. Swedish Zoological Research Expedition to Egypt and White Nile, 1901. No. 23A, pp. 1–170.Google Scholar
Pearse, A. G. E. (1961). Histochemistry Theoretical and Applied, 2nd ed. London: J. and A. Churchill Ltd.Google Scholar
Probert, A. J. (1965). Studies on the larval trematodes infecting the fresh-water molluscs of Llangorse lake S. Wales. II. The Gymnocephalous cercariae. Journal of Helminthology 39, 53–66.CrossRefGoogle Scholar
Probert, A. J. (1966). Histochemical studies on the rediae and cercariae of Echinoparyphium recurvatum Linstow. Nature, London 210, 550–1.Google Scholar
Reader, T. A. J. (1970). The biology of larval trematodes in Bithynia tentaculata (Mollusca: Gastropoda). M.Sc. thesis, University of London.Google Scholar
Sewell, R. B. S. (1922). Cercariae Indicae. Indian Journal of Medical Research 10 (suppl.), 1–370.Google Scholar
von Brand, T. (1952). Chemical Physiology of Endoparasitic Animals. New York and London: Academic Press.Google Scholar
von Brand, T. (1966). Biochemistry of Parasites. New York and London: Academic Press.Google Scholar
Wright, C. A. (1966). The pathogenesis of helminths in the Mollusca. Helminthological Abstracts 35, 207–24.Google Scholar