Hostname: page-component-848d4c4894-5nwft Total loading time: 0 Render date: 2024-06-05T01:57:41.793Z Has data issue: false hasContentIssue false
  • English
  • Français

Drug screening in cell culture for the detection of anticoccidial activity

Published online by Cambridge University Press:  06 April 2009

John F. Ryley  and
Robert G. Wilson
John F. Ryley
Affiliation:
Imperial Chemical Industries Ltd, Pharmaceuticals Division, Alderley Park, Macclesfield, Cheshire, England
Robert G. Wilson
Affiliation:
Imperial Chemical Industries Ltd, Pharmaceuticals Division, Alderley Park, Macclesfield, Cheshire, England
Get access Rights & Permissions [Opens in a new window]

Extract

Methods for screening compounds against Eimeria tenella in cell culture and in the chicken are described. An analysis for incidence of anticoccidial activity and host cell toxicity has been made of 11550 compounds screened in vitro, and some correlation of activity in vitro and in vivo has been attempted. The results show that screening compounds in tissue (cell) culture is not a satisfactory or reliable alternative to screening in chickens, although in vitro studies undoubtedly have a part to play in the evaluation of an active drug.

Type
Research Article
Information
Parasitology , Volume 73 , Issue 2 , October 1976 , pp. 137 - 148
Copyright
Copyright © Cambridge University Press 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Doran, D. J. (1970). Eimeria tenella: from sporozoites to oocysts in cell culture. Proceedings of the Helminthological Society of Washington 70, 84–92.Google Scholar
Doran, D. J. (1971). Increasing the yield of Eimeria tenella oocysts in cell culture. Journal of Parasitology 57, 891–900.CrossRefGoogle ScholarPubMed
Doran, D. J. (1973). Cultivation of coccidia in avian embryos and cell culture. In The Coccidia (ed. Hammond, D. M. and Long, P. L), pp. 183–252. Baltimore: University Park Press.Google Scholar
Fayer, R. & McLoughlin, D. K. (1970). Effect of amprolium on Eimeria adenoeides in cell culture. Journal of Parasitology 56, 388–9.CrossRefGoogle ScholarPubMed
Itagaki, K., Tsubokora, M. & Otsuki, K. (1974). Studies on methods for evaluation of anticoccidial drugs in vitro. Japanese Journal of Veterinary Science 36, 195–202.Google ScholarPubMed
Kitame, F., Utsushikawa, K., Kohama, T., Saito, T., Nikuchi, M. & Ishida, N. (1974). Laidlomycin, a new antimycoplasmal polyether antibiotic. Journal of Antibiotics 27, 884–8.CrossRefGoogle ScholarPubMed
Latter, V. S. (1973). Chemotherapeutic studies on Eimeria tenella in vitro. Parasitology 67, xv.Google Scholar
Latter, V. S. (1974). An improved in vitro chemotherapeutic screen for intracellular protozoa (Eimeria tenella and Trypanosoma cruzi). Parasitology 69, xxii–xxiii.Google ScholarPubMed
McDougald, L. R. & Galloway, R. B. (1973). Eimeria tenella: anticoccidial drug activity in cell cultures. Experimental Parasitology 34, 189–96.CrossRefGoogle ScholarPubMed
Patton, W. H. (1965). Eimeria tenella: cultivation of the asexual stages in cultured animal cells. Science 150, 767–9.CrossRefGoogle ScholarPubMed
Ryley, J. F. & Betts, M. J. (1973). Chemotherapy of chicken coccidiosis. Advances in Pharmacology and Chemotherapy 11, 221–93.CrossRefGoogle ScholarPubMed
Ryley, J. F. & Wilson, R. C. (1971). Studies on the mode of action of the coccidiostat robenidene. Zeitschrift für Parasitenkunde 37, 85–93.CrossRefGoogle ScholarPubMed
Ryley, J. F. & Wilson, R. G. (1972 a). Growth factor antagonism studies with coccidia in tissue culture. Zeitschrift für Parasitenkunde 40, 31–4.CrossRefGoogle ScholarPubMed
Ryley, J. F. & Wilson, R. G. (1972 b). Comparative studies with anticoccidials and three species of chicken coccidia in vivo and in vitro. Journal of Parasitology 58, 664–8.CrossRefGoogle ScholarPubMed
Ryley, J. F. & Wilson, R. G. (1975). Laboratory studies with some recent anticoccidials. Parasitology 70, 203–22.CrossRefGoogle ScholarPubMed
Ryley, J. F, Wilson, R. G. & Betts, M. J. (1974). Anticoccidial activity of an azauracil derivative. Parasitology 68, 69–79.CrossRefGoogle ScholarPubMed
Strout, R. G. & Ouellette, C. A. (1970). Schizogony and gametogony of Eimeria tenella in cell culture. American Journal of Veterinary Research 31, 911–18.Google Scholar
Strout, R. G. & Ouellette, C. A. (1973). Eimeria tenella: screening of chemotherapeutic compounds in cell cultures. Experimental Parasitology 33, 477–85.CrossRefGoogle ScholarPubMed
Wagenbach, G. E. (1969). Purification of Eimeria tenella sporozoites with glass bead columns. Journal of Parasitology 55, 833–8.CrossRefGoogle ScholarPubMed