Hostname: page-component-848d4c4894-75dct Total loading time: 0 Render date: 2024-05-01T02:30:54.698Z Has data issue: false hasContentIssue false
  • English
  • Français

Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents)

Published online by Cambridge University Press:  15 May 2009

F. W. A. Johnson  and
D. Hobson
F. W. A. Johnson
Affiliation:
Department of Medical Microbiology, University of Liverpool
D. Hobson
Affiliation:
Department of Medical Microbiology, University of Liverpool
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Normal non-irradiated McCoy cell cultures provide a sensitive and reproducible method for the isolation of oculo-genital strains of chlamydia A directly from human secretions and for laboratory studies with these agents. Since September 1973, chlamydia have been isolated from 175 of 562 women (32·1%) attending venereal disease clinics. Freshly isolated and low passage strains have been used to determine the importance of centrifugation, constitution and pH of the tissue culture medium, and the temperature of incubation in controlling the efficiency of plating in the method.

Type
Research Article
Information
Journal of Hygiene , Volume 76 , Issue 3 , June 1976 , pp. 441 - 451
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Bader, J. P. & Morgan, H. R. (1961). Latent viral infection of cells in tissue culture, VII. Journal of Experimental Medicine 113, 271–81.CrossRefGoogle ScholarPubMed
Becker, Y. (1974). The agent of trachoma. Monographs in Virology, vol. 7. Basle: Karger.Google Scholar
Blyth, W. A. & Taverne, J. (1974). Cultivation of TRIC agents: a comparison between the use of BHK-21 and irradiated McCoy cells. Journal of Hygiene 72, 121–8.CrossRefGoogle ScholarPubMed
Bovarnick, M. R., Miller, J. C. & Snyder, J. C. (1950). The influence of certain salts, amino-acids, sugars and proteins on the stability of rickettsiae. Journal of Bacteriology 59, 509–22.CrossRefGoogle ScholarPubMed
Croy, T. R., Kuo, C. C. & Wang, S. P. (1975). Comparative susceptibility of eleven mammalian cell lines to infection with trachoma organisms. Journal of Clinical Microbiology 1, 434–9.CrossRefGoogle ScholarPubMed
Darougar, S., Cubitt, S. & Jones, B. R. (1974). Effect of high-speed centrifugation on the sensitivity of irradiated McCoy cell culture for the isolation of chlamydia. British Journal of Venereal Diseases 50, 308–12.Google ScholarPubMed
Dunlop, E. M. C. (1975). Sticky eye in the newborn. British Medical Journal ii, 333.CrossRefGoogle Scholar
Furness, G., Graham, D. M. & Reeve, P. (1960). The titration of trachoma and inclusion blenorrhoea viruses in cell cultures. Journal of General Microbiology 23, 613–9.CrossRefGoogle ScholarPubMed
Gordon, F. B., Dressler, H. R., Quan, A. L., McQuilkin, W. T. & Thomas, J. I. (1972). Effect of ionizing irradiation on susceptibility of McCoy cultures to chlamydia trachomatis. Applied Microbiology 23, 123–9.CrossRefGoogle ScholarPubMed
Hobson, D., Johnson, F. W. A., Rees, E. & Tait, I. A. (1974). Simplified method for diagnosis of genital and ocular infections with chlamydia. Lancet ii, 555–6.CrossRefGoogle Scholar
Johnson, F. W. A. (1975). A comparison of staining techniques for demonstrating group A chlamydia in tissue culture. Medical Laboratory Technology 32, 233–8.Google Scholar
Rota, T. R. & Nichols, R. L. (1973). Chlamydia trachomatis in cell culture. Applied Microbiology 26, 560–65.CrossRefGoogle ScholarPubMed
Weiss, E. & Dressler, E. R. (1960). Centrifugation of rickettsiae and viruses onto cells and its effect on infection. Proceedings of the Society for Experimental Biology & Medicine 103, 691–5.CrossRefGoogle ScholarPubMed
Williamson, J. D. & Cox, P. (1968). Use of a new buffer in the culture of animal cells. Journal of General Virology 2, 309–12.CrossRefGoogle ScholarPubMed