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Effects of co-infection with Clonorchis sinensis on T cell exhaustion levels in patients with chronic hepatitis B

Published online by Cambridge University Press:  24 January 2024

Huimin Dong Open the ORCID record for Huimin Dong [Opens in a new window] ,
Yuan Liao ,
Mei Shang ,
Yuechun Fu ,
Hongbin Zhang ,
Minqi Luo  and
Bo Hu Open the ORCID record for Bo Hu [Opens in a new window]
Huimin Dong
Affiliation:
Department of Laboratory Medicine, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
Yuan Liao
Affiliation:
Department of Laboratory Medicine, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
Mei Shang
Affiliation:
Department of Laboratory Medicine, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
Yuechun Fu
Affiliation:
Department of Laboratory Medicine, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
Hongbin Zhang
Affiliation:
Department of Laboratory Medicine, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
Minqi Luo
Affiliation:
Department of Laboratory Medicine, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
Bo Hu*
Affiliation:
Department of Laboratory Medicine, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
*
Corresponding author: Bo Hu; Email: hubo@mail.sysu.edu.cn
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Abstract

To investigate the effects of co-infection with Clonorchis sinensis (C. sinensis) on T cell exhaustion levels in patients with chronic hepatitis B, we enrolled clinical cases in this study, including the patients with concomitant C. sinensis and HBV infection. In this study, we detected inhibitory receptors and cytokine expression in circulating CD4+ and CD8+ T cells by flow cytometry. PD-1 and TIM-3 expression levels were significantly higher on CD4+ T and CD8+ T cells from co-infected patients than on those from the HBV patients. In addition, CD4+ T cells and CD8+ T cells function were significantly inhibited by C. sinensis and HBV co-infection compared with HBV single infection, secreting lower levels of Interferon gamma (IFN-γ), Interleukin-2 (IL-2), and TNF-α. Our current results suggested that C. sinensis co-infection could exacerbate T cell exhaustion in patients with chronic hepatitis B. PD-1 and TIM-3 could be novel biomarkers for T cell exhaustion in patients with Clonorchis sinensis and chronic hepatitis B co-infection. Furthermore, it may be one possible reason for the weaker response to antiviral therapies and the chronicity of HBV infection in co-infected patients. We must realize the importance of C. sinensis treatment for HBV-infected patients. It might provide useful information for clinical doctors to choose the right treatment plans.

Keywords

Clonorchis sinensischronic hepatitis BT cell exhaustioninhibitory receptorscytokines
Type
Research Paper
Information
Journal of Helminthology , Volume 98 , 2024 , e13
Copyright
© The Author(s), 2024. Published by Cambridge University Press

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Footnotes

†

Huimin Dong and Yuan Liao contributed equally to this work.

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