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Biofilm expression and antimicrobial resistance patterns of Streptococcus uberis isolated from milk samples of dairy cows in South Africa

Published online by Cambridge University Press:  08 March 2023

Sabelo Magagula ,
Inge-Mariè Petzer ,
Ibukun Michael Famuyide  and
Joanne Karzis
Sabelo Magagula
Affiliation:
Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Onderstepoort 0110, South Africa
Inge-Mariè Petzer
Affiliation:
Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Onderstepoort 0110, South Africa
Ibukun Michael Famuyide
Affiliation:
Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Onderstepoort 0110, South Africa
Joanne Karzis*
Affiliation:
Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Onderstepoort 0110, South Africa
*
Author for correspondence: Joanne Karzis, Email: joanne.karzis@up.ac.za
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Abstract

The research described in this Research Communication addresses the hypothesis that intramammary infections with Streptococcus uberis (S. uberis) are associated with biofilm formation, which limits antibiotic efficacy. This retrospective study investigated biofilm expression and antimicrobial resistance (AMR) patterns of 172 S. uberis infections. Isolates were recovered from milk samples of subclinical, clinical, and intramammary infection cases on 30 commercial dairy herds. We determined the presence and intensity of biofilm expression of S. uberis isolates in vitro in three somatic cell count categories to recognise their AMR patterns. An automated minimum inhibitory concentration system with a commercially available panel of 23 antimicrobial agents evaluated AMR, while biofilm determination was conducted using a microplate method. The study established that all the S. uberis isolates assessed expressed biofilm with the following varying degrees of intensities: 30 (17.8%) strong, 59 (34.9%) medium and 80 (47.3%) weak biofilms. The newly registered UBAC mastitis vaccine containing biofilm adhesion components may, therefore, be a viable option for proactive mastitis management under field conditions. No differences were identified between biofilm intensity and the three somatic cell count groups. Most S. uberis isolates indicated a high-level sensitivity to the antimicrobial agents tested. Resistances were present in 8.7, 8.1 and 7.0% cases to rifampin, minocycline and tetracycline, respectively. Multidrug resistance was observed in 6.4%, emphasising AMR to antibiotics used in human medicine only. The low overall resistance suggests that farmers adhere to the prudent use of antimicrobials in the dairy industry.

Keywords

Antimicrobial resistancebiofilmdairy cowssomatic cell countStreptococcus uberis
Type
Research Article
Information
Journal of Dairy Research , Volume 90 , Issue 1 , February 2023 , pp. 42 - 46
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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References

Blignaut, D, Thompson, P and Petzer, IM (2018) Prevalence of mastitis pathogens in South African pasture-based and total mixed ration-based dairies during 2008 and 2013. The Onderstepoort Journal of Veterinary Research 85, e1–e7.CrossRefGoogle ScholarPubMed
Bolukaoto, JY, Monyama, CM, Chukwu, MO, Lekala, SM, Nchabeleng, M, Maloba, MR, Mavenyengwa, RT, Lebelo, SL, Monokoane, ST, Tshepuwane, C and Moyo, SR (2015) Antibiotic resistance of Streptococcus agalactiae isolated from pregnant women in Garankuwa, South Africa. BMC Research Notes 8, 1–7.CrossRefGoogle ScholarPubMed
Dhingra, S, Rahman, NAA, Peile, E, Rahman, M, Sartelli, M, Hassali, MA, Islam, T, Islam, S and Haque, M (2020) Microbial resistance movements: an overview of global public health threats posed by antimicrobial resistance, and how best to counter. Frontiers in Public Health 8, 531.CrossRefGoogle ScholarPubMed
Fessia, AS, Dieser, SA, Renna, MS, Raspanti, CG and Odierno, LM (2020) Relative expression of genes associated with adhesion to bovine mammary epithelial cells by Streptococcus uberis. Research in Veterinary Science 132, 33–41.CrossRefGoogle ScholarPubMed
Hsieh, JC, Yen, YS and Chuang, ST (2019) Identification of Streptococcus spp. isolated from bovine milk and characterization of their antimicrobial susceptibility profiles in Taiwan. The Thai Journal of Veterinary Medicine 49, 57–63.Google Scholar
Klaas, IC and Zadoks, RN (2018) An update on environmental mastitis: challenging perceptions. Transboundary and Emerging Diseases 65, 166–185.CrossRefGoogle ScholarPubMed
Minst, K, Märtlbauer, E, Miller, T and Meyer, C (2012) Streptococcus species isolated from mastitis milk samples in Germany and their resistance to antimicrobial agents. Journal of Dairy Science 95, 6957–6962.CrossRefGoogle ScholarPubMed
Moore, GE (2009) Biofilm production by Streptococcus uberis associated with intramammary infections. Chancellor's Honors Program Projects. Available at https://trace.tennessee.edu/utk_chanhonoproj/1299.Google Scholar
National Department of Health (2018) Surveillance resistance and consumption of antibiotics in South Africa. Department of Health. Government Publishers, Pretoria, South Africa.Google Scholar
Perovic, O and Schultsz, C (2016) Stepwise approach for implementation of antimicrobial resistance surveillance in Africa. African Journal of Laboratory Medicine 5, 7.CrossRefGoogle ScholarPubMed
Stepanović, S, Vuković, D, Hola, V, Bonaventura, GD, Djukić, S, Ćirković, I and Ruzicka, F (2007) Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. Apmis 115, 891–899.CrossRefGoogle ScholarPubMed
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