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K+ and Cl− transport by mammary secretory cell apical membrane vesicles isolated from milk

Published online by Cambridge University Press:  01 June 2009

David B. Shennan
David B. Shennan
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
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Summary

The transport of K+ (Rb+) and Cl− by membrane vesicles isolated from bovine milk has been studied using ion-exchange column chromatography. K+ (Rb+) and Cl− accumulation by the vesicles was time-dependent and was almost abolished by 0·1% Triton X-100, suggesting that uptake represents ‘real’ transport rather than binding. K+ (Rb+) uptake was influenced by the anion in solution in a manner suggesting that influx is sensitive to changes in vesicle membrane potential. Similarly, Cl− uptake was found to be sensitive to vesicle electrical potential: Cl− influx was enhanced by inside positive potentials. Cl− uptake was not saturable with respect to external Cl−. The results suggest that K+ (Rb+) and Cl− cross the apical membrane by way of conductance pathways. The similarity between ion transport by skim milk membrane vesicles and that of the apical aspect of the intact mammary epithelium suggests that the former may be a good model to study solute transport by the apical membrane of mammary secretory cells.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 3 , August 1992 , pp. 339 - 348
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

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References

REFERENCES

Blatchford, D. R. & Peaker, M. 1988 Effect of ionic composition of milk on transepithelial potential in the goat mammary gland. Journal of Physiology 402 533–541CrossRefGoogle ScholarPubMed
Christie, W. W. & Wooding, F. B. P. 1975 The site of triglyceride biosynthesis in milk. Experientia 31 1445–1447CrossRefGoogle ScholarPubMed
Furuya, K., Enomoto, K., Furuya, S., Yamagishi, S., Edwards, C. & Oka, T. 1989 Single calcium-activated potassium channels in cultured mammary epithelial cells. Pflügers Archiv 414 118–124CrossRefGoogle ScholarPubMed
Gasko, O. D., Knowles, A. F., Schertzer, H. G., Suolinna, E. M. & Racker, E. 1976 Use of ion exchange resins for studying ion transport in biological systems. Analytical Biochemistry 72 57–65CrossRefGoogle ScholarPubMed
Hunter, M. J. 1977 Human erythrocyte anion permeabilities measured under conditions of net charge transfer. Journal of Physiology 268 35–49CrossRefGoogle ScholarPubMed
Kinura, T. 1969 Electron microscopic study of the mechanism of secretion of milk. Journal of the Japanese Obstetrical and Gynaecological Society 21 301–308Google Scholar
Langridge-Smith, J. E., Field, M. & Dubinsky, W. P. 1984 Cl−-transport in apical plasma membrane vesicles isolated from bovine tracheal epithelium. Biochimica et Biophysica Acta 777 84–92CrossRefGoogle ScholarPubMed
Linzell, J. L. & Peaker, M. 1971 Mechanism of milk secretion. Physiological Reviews 51 564–597CrossRefGoogle ScholarPubMed
Mather, J. H. & Keenan, T. W. 1983 Function of endomembranes and the cell surface in the secretion of organic milk constituents. In Biochemistry of Lactation, pp. 231–283 (Ed. Mepham, T. B.). Amsterdam: Elsevier Science PublishersGoogle Scholar
Murer, H. & Kinne, R. 1980 The use of isolated membrane vesicles to study epithelial transport processes. Journal of Membrane Biology 55 81–95CrossRefGoogle ScholarPubMed
Patton, S. & Jensen, R. G. 1976 Biochemical Aspects of Lactation. Oxford: Pergamon PressGoogle Scholar
Peaker, M. & Taylor, E. 1987 Bumetanide binding to milk fat globule membrane. Journal of Physiology 386 75PGoogle Scholar
Plantz, P. E. & Patton, S. 1973 Plasma membrane fragments in bovine and caprine skim milks. Biochimica et Biophysica Acta 291 51–60CrossRefGoogle Scholar
Plantz, P. E., Patton, S. & Keenan, T. W. 1973 Further evidence of plasma membrane material in skim milk. Journal of Dairy Science 56 978–983CrossRefGoogle ScholarPubMed
Shennan, D. B. 1989 a Evidence for furosemide-sensitive (Na + K + C1) cotransport in lactating rat mammary tissue. Quarterly Journal of Experimental Physiology 74 927–938CrossRefGoogle Scholar
Shennan, D. B. 1989 b A study of sulphate transport by lactating rat mammary tissue slices: evidence for anion exchange. Comparative Biochemistry and Physiology 92A 145–150CrossRefGoogle Scholar
Shennan, D. B. 1990 Mini Review: Mechanisms of mammary gland ion transport. Comparative Biochemistry and Physiology 97 A 317–324CrossRefGoogle Scholar
Shennan, D. B. & McNeillie, S. A. 1990 Efflux of chloride from lactating rat mammary tissue slices. Comparative Biochemistry and Physiology 95 A 367–371CrossRefGoogle ScholarPubMed
Smith, K. M., McNeillie, S. A. & Shennan, D. B. 1990 K+ (Rb+) transport by a mammary secretory cell apical membrane fraction isolated from goats' milk. Experimental Physiology 75 349–358CrossRefGoogle ScholarPubMed
Wooding, F. B. P. 1974 Milk fat globule membrane material in skim-milk. Journal of Dairy Research 41 331–337CrossRefGoogle Scholar
Wooding, F. B. P., Peaker, M. & Linzell, J. L. 1970 Theories of milk secretion: evidence from the electron microscopic examination of milk. Nature 226 762–764CrossRefGoogle Scholar