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Evaluation of a rapid fluorogenic method for the detection of Escherichia coli in dairy products

Published online by Cambridge University Press:  01 June 2009

Hassan R. Sarhan ,
Leslie R. Williams  and
Howard A. Foster
Hassan R. Sarhan
Affiliation:
Department of Biological Sciences, University of Salford, Solfora M5 4WT, UK
Leslie R. Williams
Affiliation:
Department of Food and Consumer Technology, Manchester Polytechnic, Manchester M14 6HR, UK
Howard A. Foster
Affiliation:
Department of Biological Sciences, University of Salford, Solfora M5 4WT, UK
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Summary

A rapid fluorogenic medium was evaluated for the detection of Escherichia coli in dairy products. The medium was capable of detecting Esch. coli after 7·5 h incubation at 41·5 °C. Samples of pasteurized milk (136), raw milk (63), soft cheese (60) and pasteurized cream (39) were examined with media based on 4-methylumbelliferyl-β-D-glucuronide (MUG-7) and Violet red bile agar and there were no significant differences between the numbers of Esch. coli detected on the two media. MUG-7 medium had a specificity of 98·6% and the small number of organisms giving a false positive reaction were identified as Klebsiella pneumoniae. The incidence of false negative results was ∼2%. MUG-7 medium was suitable for pour plate, spread plate and membrane filtration methods. Possible applications of the method are discussed.

Type
Original Articles
Information
Journal of Dairy Research , Volume 58 , Issue 4 , November 1991 , pp. 477 - 483
Copyright
Copyright © Proprietors of Journal of Dairy Research 1991

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References

REFERENCES

American Public Health Association 1978 Standard Methods for the Examination of Dairy Products, 14th edn (Ed. Marth, E. H.). Washington, DC: APHAGoogle Scholar
American Public Health Association 1981 Standard Methods for the Examination of Water and Wastewater, 15th edn, pp. 950–952. Washington, DC: APHAGoogle Scholar
Beck, C. G. & Hehir, A. F. 1982 The rapid fluorescence method for counting bacteria in milk. Australian Journal of Dairy Technology 37 66–67Google Scholar
Chang, G. W., Brill, J. & Lum, R. 1989 Proportion of β-D-glucuronidase negative Escherichia coli in human fecal samples. Applied and Environmental Microbiology 55 335–339CrossRefGoogle ScholarPubMed
Dibb, W. L. & Bottolfsen, K. L. 1984 Evaluation of Rosco diagnostic β-glucuronidase tablets in the identification of urinary isolates of Escherichia coli. Acta Pathologica, Microbiologica et Immunologica Scandinavica Section B 92 261–264Google ScholarPubMed
Edberg, S. C. & Trepeta, R. W. 1983 Rapid and economical identification and antimicrobial susceptibility test methodology for urinary tract pathogens. Journal of Clinical Microbiology 18 1287–1291CrossRefGoogle ScholarPubMed
Feng, P. C. S. & Hartman, P. A. 1982 Fluorogenic assays for immediate confirmation of Escherichia coli. Applied and Environmental Microbiology 43 1320–1329CrossRefGoogle ScholarPubMed
Hansen, W. & Yourassowsky, E. 1984 Detection of β-glucuronidase in lactose fermenting members of the family Enterobacteriaceae and its presence in bacterial urine cultures. Journal of Clinical Microbiology 20 1177–1179CrossRefGoogle ScholarPubMed
Kilian, M. & Bülow, P. 1976 Rapid diagnosis of Enterobacteriaceae. I. Detection of bacterial glycosidases. Acta Pathologica, Microbiologica et Immunologica Scandinavica Section B 84 245–251Google Scholar
Ledford, R. A., Senyk, G. F., Shipe, W. F., Bandler, D. K. & Wolff, E. T. 1981 Rates of change in bacterial counts of commercial milks during storage. Journal of Dairy Science 64 (Suppl.) 46Google Scholar
Le Minor, L. 1979 Tetrathionate reductase, β-glucuronidase, and ONPG test in the genus Salmonella. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene Series A 243 321–325Google ScholarPubMed
Massenti, M. F., Scarlata, G. & Nastasi, A. 1981 β-Glucuronidase activity in Enterobacteriaceae. Bollettino dell'Istituto Sieroterapico Milanese Serafino Belfanti 60 26–30Google ScholarPubMed
Pérez, J. L., Berrocal, C. I. & Berrocal, L. 1986 Evaluation of a commercial β-glucuronidase test for the rapid and economical identification of Escherichia coli. Journal of Applied Bacteriology 61 541–545CrossRefGoogle ScholarPubMed
Peterkin, P. I., Sharpe, A. N. & Warburton, D. W. 1982 Inexpensive treatment of frozen dairy products for membrane filtration. Applied and Environmental Microbiology 43 486–487CrossRefGoogle ScholarPubMed
Sarhan, H. R. & Foster, H. A. 1991 A rapid fluorogenic method for the detection of Escherichia coli by the production of β-glucuronidase. Journal of Applied Bacteriology 70 (in press)CrossRefGoogle ScholarPubMed
Van Wart, M. & Moberg, L. J. 1984 Evaluation of a novel fluorogenic based method for detection of Escherichia coli. Bacteriological Proceedings 201 201Google Scholar
Weiss, L. H. & Humber, J. 1988 Evaluation of a 24-hour fluorogenic assay for the enumeration of Escherichia coli from foods. Journal of Food Protection 51 766–769CrossRefGoogle ScholarPubMed