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Superinfection inhibition by prophage B3 of some R plasmids in Pseudomonas aeruginosa

Published online by Cambridge University Press:  14 April 2009

V. Krishnapillai
V. Krishnapillai
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria 3168, Australia

Summary

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The prophage of the phage B3 of P. aeruginosa exhibits superinfection inhibition towards certain R plasmids such as R18-1 or R18-3 as shown by a severe depression (c. 10−6) in the conjugal transfer of these plasmids into B3 lysogens. The transfer of other R plasmids into such lysogens and the transfer of R18-1 or R18-3 into bacteria lysogenic for unrelated phages was unimpaired. R18-1 or R18-3 was non-transducible into B3 lysogens although transducible into non-lysogens. The interaction appeared to be reciprocally expressed since B3 lysogenization of bacteria R+ for R18-1 or R18-3 was also reduced. Consistent with the failure of co-existence of the prophage and the plasmids was the observation that when the transconjugants from the cross R18-1 donor ×B3 lysogenic recipients were tested, transfer-deficient plasmid derivatives were encountered (about 40%). Other transconjugants were cured of prophage, or carried mutant B3 prophage with lytic, lysogenization or superinfection inhibition functions impaired.

Type
Research Article
Information
Genetics Research , Volume 29 , Issue 1 , February 1977 , pp. 47 - 54
Copyright
Copyright © Cambridge University Press 1977

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