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Influence of fructose feeding on individual enzymic reactions in the formation and metabolism of bile acids in rat liver homogenates

Published online by Cambridge University Press:  10 January 2017

M. Carrella ,
I. Björkhem ,
K. Einarsson  and
K. Hellström
M. Carrella
Affiliation:
Department of Medicine, Serafimerlasarettet and Department of Chemistry, Karolinska Institutet, Stockholm, Sweden
I. Björkhem
Affiliation:
Department of Medicine, Serafimerlasarettet and Department of Chemistry, Karolinska Institutet, Stockholm, Sweden
K. Einarsson
Affiliation:
Department of Medicine, Serafimerlasarettet and Department of Chemistry, Karolinska Institutet, Stockholm, Sweden
K. Hellström
Affiliation:
Department of Medicine, Serafimerlasarettet and Department of Chemistry, Karolinska Institutet, Stockholm, Sweden
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1. Rats were maintained for 10 d on a semi-synthetic diet containing 700 g glucose or 700 g fructose/kg. Individual enzymie reactions in bile acid synthesis and metabolism were studied by measuring the 7α-hydroxylation of [4-14C]cholesterol, the 12α-hydroxylation of 7α-[6β-3H]hydroxy-4-cholesten-3-one, the 26-hydroxylation of 5β-[7β-3H]cholestane-3α,7α-diol and the 6β-hydroxylation of [3H]lithocholic acid in liver homogenates.

2. The serum cholesterol level was approximately the same in both groups of animals, but the serum triglyceride level was almost twice as high in the fructose-fed rats compared to the glucose-fed rats.

3. The 6β-hydroxylation of [3H]lithocholic acid was increased by about 20% in the fructose-fed rats compared to the glucose-fed animals. The activities of the other enzymic reactions studied did not differ significantly between the two groups of animals. The findings are discussed in relation to previous knowledge concerning mechanisms regulating triglyceride, pre-β-lipoprotein and bile acid synthesis.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 36 , Issue 2 , September 1976 , pp. 273 - 279
Copyright
Copyright © The Authors 1976

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