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Chapter 32 - Survival of Primordial Follicles

Tips and Tricks

from Section 8 - In Vitro Follicle Culture

Published online by Cambridge University Press:  27 March 2021

By
Christiani A Amorim  and
Carolina M Lucci
Edited by
Jacques Donnez  and
S. Samuel Kim
Jacques Donnez
Affiliation:
Catholic University of Louvain, Brussels
S. Samuel Kim
Affiliation:
University of Kansas School of Medicine
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Summary

According to the European Society of Human Reproduction and Embryology (ESHRE) Working Group on Oocyte Cryopreservation [], between 2010 and 2014, cancer patients in Europe underwent similar numbers of ovarian tissue cryopreservation procedures (~4,400) and oocyte cryopreservation procedures (~3,800). This milestone in the field of ovarian tissue cryopreservation is responsible for a new surge of interest in primordial follicles and their requirements for survival and development, as they represent more than 90% of the follicle population to be cryopreserved. When patients cannot have their frozen-thawed ovarian tissue reimplanted due to the risk of transmission of malignant cells, these follicles can be isolated and grafted inside a bioengineered ovary (see Chapter 36) or grown in vitro (see Chapter 31). We will focus on the latter strategy, more specifically on the main elements that primordial follicles need to survive in vitro.

Type
Chapter
Information
Fertility Preservation
Principles and Practice
 , pp. 364 - 380
Publisher: Cambridge University Press
Print publication year: 2021

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References

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