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Expression of miR-145 and miR-18b in Peripheral Blood Samples of Head and Neck Cancer Patients

  • ORIGINAL RESEARCH ARTICLE
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Indian Journal of Clinical Biochemistry Aims and scope Submit manuscript

Abstract

Head and neck squamous cell carcinomas (HNSCC) is one of the most prevalent type of cancer known in Indian population. Studies are needed to identify the early biomarkers for HNSCC. MicroRNAs (miRNAs) are non-coding RNA molecules, expression of which can be used as biomarker for early diagnosis of HNSCC. For miRNA profiling total RNA, which also contained small RNAs were isolated from ten HNSCC tissue samples and adjacent control. Purity and concentration of eluted RNA was assessed using the NanoDrop1000® spectrophotometer, Reverse Transcription reaction was carried out with megaplex RT primers of pool A and pool B and the expression of selected miRNAs (miR-143/145 and miR-18a/b) was measured using TaqMan primers specific for mature miRNAs. Our study showed dramatic downregulation in expression of two miRNAs, miR-18b and miR-145 in blood samples of HNSCC patients, which are inhibitor of tumorigenesis and can be targeted as biomarker of HNSCC pathogenesis therefore developing avenues for miRNA role in prognosis and therapeutics.

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Data availability

All data generated or analysed during this study are included in this article (and its supplementary information files). All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest or non-financial interest in the subject matter or materials discussed in this manuscript.

Abbreviations

HNSCC:

Head and neck squamous cell carcinomas

miRNAs:

MicroRNAs

RNA:

Ribonucleic acid

HNC:

Head and neck cancer

CT:

Cycle threshold

ROC:

Receiver operating characteristic

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Acknowledgments

Authors acknowledge Council of Scientific and Industrial Research (CSIR) for funding the work carried out in the present study through InDepth project (BSC0111). Nishant Singh and Sanjeev Kumar Yadav is grateful CSIR, New Delhi, for providing JRF/SRF fellowship.

Author information

Authors and Affiliations

  1. Department of Radiotherapy, Sarojini Naidu Medical College, Moti Katra, Agra, Uttar Pradesh, 282002, India

    Arun Kumar Yadav

  2. Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India

    Nishant Singh

  3. Developmental Toxicology Laboratory, Systems Toxicology and Health Risk Assessment Group, CSIR - Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31 Mahatma Gandhi Marg, Lucknow, Uttar Pradesh, 226001, India

    Sanjeev Kumar Yadav

  4. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Sanjeev Kumar Yadav

  5. King Georges’ Medical University, Lucknow, Uttar Pradesh, India

    M. L. B. Bhatt

  6. Apollo Medics, Lucknow, Uttar Pradesh, India

    Amit Pandey

  7. Moti Lal Nehru Medical College, Prayagraj, Uttar Pradesh, India

    Dev Kumar Yadav

  8. All India Institute of Medical Sciences (AIIMS), Raebareli, Uttar Pradesh, 229405, India

    Sanjay Yadav

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  1. Arun Kumar Yadav
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Contributions

Conceptualization: [Arun Kumar Yadav, MLB Bhatt, Sanjay Yadav]; Methodology: [Arun Kumar Yadav, Sanjay Yadav]; Formal analysis and investigation: [Arun Kumar Yadav, Nishant Singh, Sanjeev Kumar Yadav, Amit Pandey, Dev Kumar Yadav], Writing – original draft preparation: [Arun Kumar Yadav, Nishant Singh, Sanjeev Kumar Yadav, Sanjay Yadav]; Writing - review and editing: [Arun Kumar Yadav, Nishant Singh, Sanjeev Kumar Yadav, Sanjay Yadav], Supervision: [MLB Bhatt, Sanjay Yadav].

Corresponding author

Correspondence to Arun Kumar Yadav.

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The study was approved by the Institutional Ethics committee of King George’s Medical University (KGMU) U.P., India (1667/Ethics/R.cell-17). The methods employed in this investigation follow the guidelines laid out in the Declaration of Helsinki.

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A written informed consent was obtained from the study participant or legal guardian and was explained about the study procedure.

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Yadav, A.K., Singh, N., Yadav, S.K. et al. Expression of miR-145 and miR-18b in Peripheral Blood Samples of Head and Neck Cancer Patients. Ind J Clin Biochem 38, 528–535 (2023). https://doi.org/10.1007/s12291-023-01119-2

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  • DOI: https://doi.org/10.1007/s12291-023-01119-2

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