Abstract
Hypoxia is a common phenomenon in aquaculture. With the dissolved oxygen (DO) 3.75 ± 0.25 mg O2 /L for hypoxia group and 7.25 ± 0.25 mg O2 /L for control group for 30, 60, and 90 days, long-term hypoxia stress was used to investigate the oxidative stress, apoptosis, and immunity in the intestine of Pelteobagrus vachelli. According to the results of measurement of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-PX), and catalase (CAT) activities and malondialdehyde (MDA) content, the oxidative stress ability of the intestine was activated at 30 days and impaired at 60 and 90 days. The upregulation of Bcl-2-associated x (Bax); downregulation of B cell lymphoma-2 (Bcl-2); increased activities of caspase-3, caspase-9, and Na+-K+-ATPase; decreased activities of succinate dehydrogenase (SDH); and the release of cytochrome c (Cyt-c) in mitochondria revealed that hypoxia induced the apoptosis. Moreover, heat shock protein 70 (HSP 70), heat shock protein 90 (HSP 90), immunoglobulin M (IgM), and C-lysozyme (C-LZM) were activated to inhibit apoptosis, but the immunoregulatory function might be damaged at 60 and 90 days. This study provides a theoretical foundation for understanding the mechanisms of hypoxia stress and aquaculture management of P. vachelli.
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This study was supported by the “JBGS” Project of Seed Industry Revitalization in Jiangsu Province [JBGS(2021)034]; the Creation Project of Major New Species of Agriculture in Jiangsu Province (PZCZ201742); and Jiangsu Province “333 High-level Talents Cultivating Project.”
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Tao Wang conceived this study and designed and supervised the experiments; Min Wang, Shujia Liao, Xuechun Zang, and Zhineng Fu performed the experiments, conducted data analysis, prepared figures and tables, and wrote the manuscript. Tao Wang and Shaowu Yin modified the manuscript. All authors reviewed and approved the manuscript.
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Wang, M., Liao, S., Zang, X. et al. Long-term hypoxia stress-induced oxidative stress, cell apoptosis, and immune response in the intestine of Pelteobagrus vachelli. Fish Physiol Biochem 49, 585–597 (2023). https://doi.org/10.1007/s10695-023-01204-6
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DOI: https://doi.org/10.1007/s10695-023-01204-6