Abstract
A tandem-column liquid chromatographic method was developed to separate ten non-steroidal anti-inflammatory drugs (Aceclofenac, Aspirin, Carprofen, Celecoxib, Diclofenac, Flurbiprofen, Ibuprofen, Ketoprofen, Naproxen, Salicylic Acid) using an achiral tandem-column configuration under reversed-phase conditions. An NSAID test mix was prepared with 0.5 mg/mL thiourea as a t0 marker and 0.5 mg/mL of the above NSAIDs in 1:1 acetonitrile: 0.2% formic acid. Three different 5-cm columns were investigated individually and in two different tandem-column configurations: an octyl (C8) column, a perfluorophenyl (PFP) column, and a biphenyl (BP) column. The two tandem configurations investigated were a PFP column followed by either a BP or C8 column. While none of the individual columns provided the selectivity needed to separate two sets of critical pairs (Ketoprofen and Naproxen; Flurbiprofen and Ibuprofen), the unique selectivity provided by the two tandem-column combinations enabled both critical pairs to be separated. The PFP + BP tandem column combination was able to separate the 10 NSAIDs with baseline or greater resolution in less than 25 min.
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The authors would like to thank Restek for providing the columns, and Drexel University for the teaching fellowship awarded to MEM.
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Drexel University, Department of Chemistry, Restek RASP grant.
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M.E.M. conducted the experiments, wrote the first draft of the main manuscript text, participated in subsequent revisions of the main text, provided Figures 1-3, and supplied the first draft of the Supplemental Information. J.P.F. mentored M.E.M on all aspects of this research, performed considerable revision of the main manuscript and Supplemental Information, and provided the final drafts.
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Marrazzo, M.E., Foley, J.P. Tandem-Column High Performance Liquid Chromatography Separation of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs). Chromatographia 86, 743–750 (2023). https://doi.org/10.1007/s10337-023-04286-9
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DOI: https://doi.org/10.1007/s10337-023-04286-9