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Photobiomodulation and Sida tuberculata combination declines the inflammation’s markers in knee-induced osteoarthritis

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Abstract

The aim of this study was to assess potential combination effects of photobiomodulation therapy (PBMT) with Sida tuberculata extracts on the oxidative stress and antioxidant activity, as well as on the inflammatory process. Rats with knee osteoarthritis (OA) were treated with S. tuberculata extracts and PBMT (904 nm, 18 J/cm2). The animals were evaluated for nociception and edema. The blood, knee lavage and structures, spinal cord, and brainstem were collected for biochemical analyses (lipid peroxidation, protein carbonyl content, superoxide dismutase activity, non-protein thiol levels, and measurement of nitrite/nitrate). The knee structures were also used to measure cytokine levels. PBMT lowered the damage due to oxidative stress in the knee and at distant sites from the lesion. PBMT also reduced the levels of nitric oxide and cytokines, which could explain the nociception reduction mechanism. Similarly, S. tuberculata decreased the damage by oxidative stress, levels of nitrite/nitrate, and cytokines. The therapy combination reduced levels of cytokines and nitrite/nitrate. PBMT and S. tuberculata extracts reduced the oxidative stress and inflammation. It is noteworthy that PBMT increased the antioxidant activity in the knee and at sites distant from the lesion, contributing to a more significant decrease in nociception. The combination of therapies did not present significant effects on the analyzed parameters. Therefore, it is suggested that PBM is sufficient to minimize the signs and symptoms of the knee OA in our rat model.

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Funding

This work was partially supported by CNPq and FAPESC, research-funding agencies in Brazil.

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Authors and Affiliations

  1. Graduate Program in Biochemistry, Universidade Federal do Pampa (Unipampa), BR 472, Km 585, Uruguaiana, RS, 97501-970, Brazil

    Eloá Ferreira Yamada, Vanderlei Folmer & Morgana Duarte da Silva

  2. Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analyses, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil

    Carolina dos Santos Stein & Rafael Noal Moresco

  3. Graduate Program in Health Sciences, Laboratory of Experimental Neuroscience (LaNEx), Universidade do Sul de Santa Catarina (UNISUL), Palhoça, SC, 88137-272, Brazil

    Franciane Bobinski, Juliete Palandi & Paula Franson Fernandes

  4. Laboratory of Experimentation in Neuropathology (LEN), Graduate Program in Neuroscience, Biochemistry Department, Biological Sciences Center, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil

    Juliete Palandi

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  1. Eloá Ferreira Yamada
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Contributions

Yamada EF: Idea and experimental design, acquisition of data, data verification and interpretation, writing of the manuscript, critical revision, and final approval of the article

Stein CS: Collection and assembly of data and final approval of the article

Moresco RN: Data analyses and interpretation and final approval of the article

Bobinski F: Data analyses and interpretation and final approval of the article

Palandi J: Data collection and assembly and final approval of the article

Fernandes PF: Data collection, revision, and final approval of the article

Folmer V: Idea and experimental design, manuscript critical revision, and final approval of the article

Da Silva MD: Idea and experimental design, acquisition of data, data verification and interpretation, writing of the manuscript, manuscript critical revision, and final approval of the article

Corresponding author

Correspondence to Eloá Ferreira Yamada.

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The authors declare that they have no conflict of interest.

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The study were approved by the Institutional Ethics Committee of the Universidade Federal do Pampa (RS/Brazil), protocol 004/2016, and were performed following the “Guidelines for Integrity and Good Practices for the Production, Maintenance or Use of Animals” from the CONCEA (Conselho Nacional de Controle de Experimentação Animal) resolution (no. 32, 09/2016).

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Yamada, E.F., dos Santos Stein, C., Moresco, R.N. et al. Photobiomodulation and Sida tuberculata combination declines the inflammation’s markers in knee-induced osteoarthritis. Lasers Med Sci 37, 193–204 (2022). https://doi.org/10.1007/s10103-020-03207-8

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