Abstract
Four halophilic archaea strains, AD-4T, CGA30T, CGA73T, and WLHSJ27T, were isolated from a salt lake and two soda lakes located in different regions of China. The 16S rRNA and rpoB′ gene sequence similarities among strains AD-4T, CGA30T, CGA73T, WLHSJ27T, and the current species of the family Natrialbaceae were 90.9–97.5% and 83.1–91.8%, respectively. The phylogenetic and phylogenomic analyses revealed that these four strains separated from existing genera in the family Natrialbaceae and formed distant branches. The ANI, isDDH, and AAI values among these four strains and the current members of the family Natrialbaceae were 72–79%, 20–25%, and 63–73%, respectively, much lower than the threshold values for species demarcation. Strains AD-4T, CGA73T, and WLHSJ27T may represent three novel genera of the family Natrialbaceae according to the cutoff value of AAI (≤ 76%) proposed to differentiate genera within the family Natrialbaceae. These four strains could be distinguished from the related genera according to differential phenotypic characteristics. The major phospholipids of these four strains were identical while their glycolipid profiles were diverse. DGD-1 is a major glycolipid found in strain AD-4T, trace glycolipids, DGD-1, and S-DGD-1, and (or) S-TGD-1 was found in the other three strains. The major respiratory quinones detected in the four strains were menaquinone MK-8 and MK-8(H2). This polyphasic classification indicated that strains AD-4T, CGA73T, and WLHSJ27T represent three novel species of three new genera with the family Natrialbaceae, and strain CGA30T represents a novel species of Halovivax.
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The sequences determined in this study were deposited and available in the NCBI GenBank.
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Acknowledgements
We are grateful to the graduated students (Qin Xu, Yang-Jie Zhao, and Cong-Qi Tao, Master’s degree), for their primary contribution to this study.
Funding
This work was financially supported by the National Natural Science Foundation of China (No. 32070003), and the National Science and Technology Fundamental Resources Investigation Program of China (No. 2019FY100700; No. 2021FY100900).
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Li, XX., Tan, S., Cheng, M. et al. Salinilacihabitans rarus gen. nov., sp. nov., Natrononativus amylolyticus gen. nov., sp. nov., Natronobeatus ordinarius gen. nov., sp. nov., and Halovivax gelatinilyticus sp. nov., halophilic archaea, isolated from a salt lake and soda lakes. Extremophiles 27, 15 (2023). https://doi.org/10.1007/s00792-023-01303-5
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DOI: https://doi.org/10.1007/s00792-023-01303-5