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A rooting procedure for lentil (Lens culinaris Medik.) and other hypogeous legumes (pea, chickpea and Lathyrus) based on explant polarity

  • Cell Biology and Morphogenesis
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Abstract

The present study assessed the rooting response of lentil nodal segments in relation to explant polarity, hormone, salt and carbohydrate concentrations of the medium. Nodal segments of lentil with an axillary bud cultured in an inverted orientation (apical end in medium) showed higher rooting frequencies than explants cultured in a normal orientation (basal end in medium). The highest rooting percentage (95.35%) and average number of shoots regenerated per explant (2.4) were obtained from explants placed in an inverted orientation on Murashige and Skoog (MS) medium salts with 3% sucrose, supplemented with 5 μM indole acetic acid (IAA) and 1 μM kinetin (KN). Reducing or increasing phytohormone concentration did not alter significantly root regeneration of inverted explants. Sucrose at 3% allowed higher root regeneration frequencies compared to 1.5% sucrose. MS full concentration permitted regeneration of longer shoots with more nodes per regenerated shoot, compared to MS half-strength, which regenerated more shoots of shorter length and with less nodes. Inverted nodal segments of other hypogeous legumes (pea, chickpea and Lathyrus) also exhibited higher rooting frequencies than explants cultured in a normal orientation on MS medium with 3% sucrose and supplemented with 5 μM IAA and 1 μM KN. The most novel application of this study is the culture of nodal segments of hypogeous legumes in an inverted orientation. This procedure is a considerable improvement over other published procedures concerning in vitro rooting of lentil, pea, chickpea and Lathyrus.

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Fig. 1A–F.

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Acknowledgements

This work was supported by the Spanish DGICYT grant AGF99-0192 and by the Junta de Castilla y León grant LE21/01, and a personal Ph.D. grant to R. Fratini from the University of León.

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Authors and Affiliations

  1. Area de Genética, Facultad de Biología, Universidad de León, 24071, León, Spain

    R. Fratini & M. L. Ruiz

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  1. R. Fratini
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  2. M. L. Ruiz
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Correspondence to M. L. Ruiz.

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Communicated by D. Bartels

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Fratini, R., Ruiz, M.L. A rooting procedure for lentil (Lens culinaris Medik.) and other hypogeous legumes (pea, chickpea and Lathyrus) based on explant polarity. Plant Cell Rep 21, 726–732 (2003). https://doi.org/10.1007/s00299-003-0603-z

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  • DOI: https://doi.org/10.1007/s00299-003-0603-z

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