Summary
This study presents the first comparative analysis of the effect of four different cytokinins, applied to different lentil (Lens culinaris Medik.) explants in four different concentrations. with regard to the regeneration of shoots and roots of the pulse crop. The variables explant, phytohormone and concentration were all found to be highly significant. Mature seed explants showed the highest shoot regeneration over all the phytohormones and concentrations tested. Thidiazuron (TDZ) and benzyladenine (BA) showed a higher number of regenerated shoots than kinetin (KIN) and zeatin (ZEA); an increase from 1.25 μM to 10 μM of any cytokinin in general doubled the number of shoots regenerated. The average length of regenerated shoots was found to be inversely proportional to the number of shoots regenerated. TDZ and BA were found to inhibit root development more than KIN and ZEA. The highest root regeneration frequency was obtained from shoots regenerated on media containing 1.25 μM ZEA. The study concludes that in order to obtain whole plants it is best to regenerate shoots on media containing the cytokinins KIN or ZEA at low concentrations, in order to be able subsequently to regenerate roots.
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Ahmad, M.; Fautrer, A. G.; McNeil, V.; Hill, G. D.; Burritt, D. J. In vitro propagation of Lens species and their F1 interspecific hybrids. Plant Cell Tiss. Organ Cult. 47:169–176; 1997.
Akasaka, Y.; Daimon, H.; Mii, M. Improved plant regeneration from cultured leaf segments in peanut (Arachis hypogaea L) by limited exposure to thidiazuron. Plant Sci. 156:169–175; 2000.
Bajaj, Y. P. S.; Dhanju, M. S. Regeneration of plants from apical meristem tips of some legumes. Curr. Sci. 48:906–907; 1979.
Berleth, T.; Mattsson, J. Vascular development: tracing signals along veins. Curr. Opin. Plant Biol. 3:406–411; 2000.
Böhrner, P.; Meyer, B.; Jacobsen, H. J. Thidiazuron induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus. Plant Cell Rep. 15:26–29; 1995.
Brzobohaty, B.; Moore, I.; Palme, K. Cytokinin metabolism: implications for regulation of plant growth and development. Plant Mol. Biol. 26:1483–1497; 1994.
Costa, S.; Dolan, L. Development of the root pole and cell patterning in Arabidopsis roots. Curr. Opin. Genet. Dev. 10:405–409; 2000.
Fernandez, M. Cultivo in vitro de lenteja: estudio de la inducción y regeneración de plantas. Tesina de Licenciatura, Universidad de León, Spain; 1995.
Gulati, A.; Jaiwal, P. K. Plant regeneration from cotyledonary node explants of mungbean (Vigna radiata (L.) Wilczek) Plant Cell Rep. 13:523–527; 1994.
Ladizinky, G. The origin of lentil and its wild gene pool. Euphytica 28:179–187; 1979.
Malik, K. A.; Saxena, P. K. Thidiazuron induces high frequency shoot regeneration in intact seedlings of pea (Pisum sativum), chickpea (Cicer arietinum) and lentil (Lens culinaris). Aust. J. Plant Physiol. 19:731–740; 1992.
Mohamed, M. F.; Read, P. E.; Coyne, D. P. Plant regeneration from in vitro culture of embryonic axis explants in common and tepary beans. J. Am. Soc. Hort. Sci. 117 (2):332–336; 1992.
Mohan, M. L.; Krishnamurthy, K. V. Plant regeneration in pigconpea [Cajanus cajan (L.) Millsp.] by organogenesis. Plant Cell Rep. 17:705–710; 1998.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Ocampo, B.; Conicella, C.; Moss, J. P. Wide crossing: opportunities and progress. In: Knight, R., ed. Linking research and marketing opportunities for pulses in the 21st century. Proceedings of the Third International Food Legumes Research Conference. Current plant science and biotechnology in agriculture, vol. 34. Dordrecht, Boston London, Kluwer Academic Publishers; 2000: 411–419.
Polanco, M. C. Estudio de la regeneración in vitro de plantas de lenteja (Lens culinaris Medik) y análisis de las plantas regeneradas. PhD dissertation, Universidad de León, Spain; 1995.
Polanco, M. C.; Pelaez, M. I.; Ruiz, M. L. Factors affecting callus, and shoot formation from in vitro cultures of Lens culinaris Medik. Plant Cell Tiss. Organ Cult. 15:175–182; 1988.
Polanco, M. C.; Ruiz, M. L. Effect of benzylaminopurine on in vitro and in vivo root development in lentil (Lens culinaris Medik.) Plant Cell Rep. 17:22–26; 1997.
Polanco, M. C.; Ruiz, M. L. Factors that affect plant regeneration from in vitro culture of immature seeds in four lentil (Lens culinaris Medik.) cultivars. Plant Cell Tiss. Organ Cult. 66:133–139; 2001.
Polisetty, R.; Paul, V.; Deveshwar, J. J.; Khetarpal, S.; Suresh, K.; Chandra, R. Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.). Plant Cell Rep. 16:565–571; 1997.
Prakash, S. N.; Pental, D.; Sarin, N. B. Regeneration of Pigeonpea (Cajanus cajan) from cotyledonary node via multiple shoot formation. Plant Cell Rep. 13:623–627; 1994.
Sanago, M. H. M.; Shattuck, V. I.; Strommer, J. Rapid plant regeneration of pea using thidiaruzon. Plant Cell Tiss. Organ Cult. 45:165–168; 1996.
Saxena, P. K.; King, J. Morphogenesis in lentil: plant regeneration from callus cultures of Lens culinaris Medik. via somatic embryogenesis. Plant Sci. 52:223–227; 1987.
Selva, E.; Stouffs, B.; Briquet, M. In vitro propagation of Vicia faba L. by micro-cutting and multiple shoot induction. Plant Cell Tiss. Organ Cult. 18:167–179; 1989.
Singh, R. K.; Raghuvanshi, S. S. Plantlet regeneration from nodal segment and shoot tip derived explants of lentil. Lens Newslett 16:33–35; 1989.
Subhadra; Vahishat, R. K.; Chowdhury, J. B.; Singh, M.; Sareen, P. K. Multiple shoots from cotyledonary node explants of non-nodulating genotype (ICC435M) of chickpea, Cicer arietinum L. Indian J. Exp. Biol. 36:1276–1279; 1998.
Williams, D. J.; McHughen, A. Plant regeneration of the legume lens culinaris Medik (lentil) in vitro. Plant Cell Tiss. Organ Cult. 7:149–153; 1986.
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Fratini, R., Ruiz, M.L. Comparative study of different cytokinins in the induction of morphogenesis in lentil (Lens culinaris Medik). In Vitro Cell.Dev.Biol.-Plant 38, 46–51 (2002). https://doi.org/10.1079/IVP2001252
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DOI: https://doi.org/10.1079/IVP2001252