Abstract
A novel Gram-staining-negative and short-rod-shaped bacterial strain designated as 1NDH52T was isolated from a tidal flat sediment and characterized using a polyphasic taxonomic approach. The predominant cellular fatty acids of strain 1NDH52T were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and C14:0 2-OH; the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid; the major respiratory quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1NDH52T was closely related to type strains Croceicoccus sediminis S2-4-2 T (98.9%), Croceicoccus bisphenolivorans H4T (98.9%) and Croceicoccus pelagius Ery9T (98.7%). Phylogenomic analysis indicated that strain 1NDH52T formed an independent branch distinct from all type strains of this genus. The overall genome related indices including the digital DNA–DNA hybridization values, average nucleotide identities and average amino acid identities between strain 1NDH52T and the three close relatives above indicated that strain 1NDH52T should represent a novel genospecies. The genomic DNA G + C content was 62.6%. Strain 1NDH52T could produce carotenoids and its genome contained the complete carotenoids biosynthetic gene cluster. Based on the phenotypic and genotypic characteristics, strain 1NDH52T is concluded to represent a novel species of the genus Croceicoccus, for which the name Croceicoccus gelatinilyticus sp. nov., is proposed. The type strain of the species is 1NDH52T (= GDMCC 1.2381 T = KCTC 82668 T).
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Acknowledgements
We are very grateful for Mr. Fan Yang at Institute of Microbiology, Guangdong Academy of Sciences (Guangzhou, China) for purchasing type strains.
Funding
This work was jointly supported by the Key-Area Research and Development Program of Guangdong Province (2018B020205003), the GDAS' Project of Science and Technology Development (2020GDASYL-20200103028) and the Science and Technology Plan Project of Guangdong Province (2019B030316017).
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Pei, T., Liu, Y., Du, J. et al. Croceicoccus gelatinilyticus sp. nov., isolated from a tidal flat sediment. Arch Microbiol 204, 93 (2022). https://doi.org/10.1007/s00203-021-02703-x
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DOI: https://doi.org/10.1007/s00203-021-02703-x