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Influence of exogenous DNA on Ypenyl-treated chromosomes ofVicia faba L.

Vliv exogenní desoxyribonukleové kyseliny na Ypenylem poškozené chromosomyVicia faba

Влияние экзогенной дезоксирибонуклеиновой кислоты на хромозомыVicia faba поврежденные Ипенилом

  • Published:
Biologia Plantarum

Abstract

This paper is a study of the effect of exogenous DNA of different genetic origins on the repair of meristematic cells of primary roots ofVicia faba, damaged by 24 hour treatment with 0·01mm solution of Ypenyl. Both kinds of DNA,i.e. isologous and heterologous, stimulated cell proliferation which was decreased by the action of the radiomimetic and influenced both dynamics of production of chromosome aberrations and the interchromosomal distribution of induced damage. While heterologous DNA increased the frequency of aberrations after all recovery periods studied, isologous DNA significantly decreased the number of chromosomal aberrations. Heterologous DNA increased at the same time the relative number of breaks in the group of small chromosomes, while by the action of isologous DNA the number of aberrations related to this group of chromosomes was relatively decreased.

Abstract

Byl studován účinek exogenní desoxyribonukleové kyseliny různého genetického původu na reparaci meristematických buněk primárního kořeneVicia faba. Obě DNK, tj. isologní i heterologní, urychlily buněčnou proliferaci, sníženou působením radiomimetika, a ovlivnily jak dynamiku tvorby chromosomových aberací, tak interchromosomální distribuci indukovaných poruch. Zatím co heterologní DNK ve všech studovaných časových intervalech zotavení zvýšila zároveň frekvenci chromosomových aberací, isologní DNK projevila efekt reparační, takže významně procento aberací snížila. Heterologní DNK současně zvýšila počet poškození ve skupině malých chromosomů, isologní DNK naopak počet abnormalit v této skupině relativně snížila.

Abstract

Исследовалось влияние извне подапной дезоксирибонуклеиновой кислоты разного генетического происхождения на возобновительные процессы в меристематических клетках первичного корняVicia faba поврежденных 24-х часовым действием 0,01 мМол раствором Ипенила. Обе ДНК, т. е. изологная и гетерологная, ускорили процесс пролиферации клеток, замедленный под действием радиомиметического вещества и повлияли на динамику образования хромосомных аберраций а также на межхромосомальное распределение вызванных повреждений. Гетерологная ДНК во всех исследованных промежутках времени восстановления от повреждения повышала частотность хромосомных аберраций, изологная ДНК имела восстановительный эффект и значимо понижала процент аберраций. Гетерологная ДНК наряду с этим повысила количество повреждений в группе малых хромосом. Изологная ДНК напротив в данной группе количество аномалий относительно понизила.

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  1. Biophysical Institute, Czechoslovak Academy of Sciences, Czechoslovakia

    Jana Šlotová & Z. Karpfel

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  1. Jana Šlotová
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  2. Z. Karpfel
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Address: Královopolská 135, Brno, Czechoslovakia.

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Šlotová, J., Karpfel, Z. Influence of exogenous DNA on Ypenyl-treated chromosomes ofVicia faba L.. Biol Plant 11, 216–225 (1969). https://doi.org/10.1007/BF02920800

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  • DOI: https://doi.org/10.1007/BF02920800

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