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Genomic Tools Used in Molecular Clinical Aging Research

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Clinical Genetics and Genomics of Aging

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Abstract

The development of next-generation sequencing (NGS) technologies to study the genome, the transcriptome, and the epigenome has revolutionized the research in all life sciences. Whole-genome sequencing and related emerging techniques are changing the perspective of researchers about the complexity of all biological phenomena. Now, the interaction between molecules and their cellular functions can be studied with high-throughput methods and in a relatively easy way, although most technologies are still expensive. In this regard, this era of post-genomic studies needs to be the time for our better understanding of the aging process and related diseases. Moreover, these technologies are a major promise to catapult biomedical research into true clinical applications as prognostics or even more as therapeutic tools for many human conditions such as aging and associated diseases. In this chapter, we reviewed how different NGS strategies have been used in the study of longevity, aging, and age-related diseases. These NGS strategies include exome and whole-genome sequencing, transcriptome sequencing, single-cell whole-genome sequencing, mitochondrial genome sequencing, DNA methylation sequencing, and ChIP-seq.

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Abbreviations

ChIP:

Chromatin immunoprecipitation

ChIP-seq:

ChIP sequencing

DMR:

Differentially methylated regions

DNA:

Deoxyribonucleic acid

DNAmet:

DNA methylation

FACS:

Fluorescence-activated cell sorting

LCM:

Laser capture microdissection

mtDNA:

Mitochondrial DNA

NGS:

Next-generation sequencing

RNA:

Ribonucleic acid

RNA-seq:

RNA sequencing

scRNA-seq:

Single-cell RNA-seq

SCS:

Single-cell sequencing

SMS:

Single-molecule sequencing

WES:

Whole-exome sequencing

WGS:

Whole-genome sequencing

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Author information

Authors and Affiliations

  1. Epigenetics Laboratory, Instituto Nacional de Medicina Genómica, Mexico City, Mexico

    Alfredo García-Venzor

  2. Laboratory of Connective Tissue, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra Ibarra”, Mexico City, Mexico

    Edna Ayerim Mandujano-Tinoco

Authors
  1. Alfredo García-Venzor
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  2. Edna Ayerim Mandujano-Tinoco
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Editors and Affiliations

  1. Basic Sciencies Division, National Institute of Geriatrics, Ciudad de México, Mexico

    Juan Carlos Gomez-Verjan

  2. Instituto Nacional de Geriatría (INGER), Ciudad de México, Mexico

    Nadia Alejandra Rivero-Segura

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García-Venzor, A., Mandujano-Tinoco, E.A. (2020). Genomic Tools Used in Molecular Clinical Aging Research. In: Gomez-Verjan, J., Rivero-Segura, N. (eds) Clinical Genetics and Genomics of Aging. Springer, Cham. https://doi.org/10.1007/978-3-030-40955-5_5

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