Skip to main content
SpringerLink
Log in

Conformation-Dependent Recognition Sites

  • Chapter
Integrin-Ligand Interaction

Abstract

The integrin-mediated interaction of cells with laminins has recently been reviewed by Aumailley et al.1 In this chapter, the structural aspects of laminin integrin interaction will be emphasized. At least seven integrins have been identified which bind to laminin (Fig. 4.3.1). They were determined by using inhibiting antibodies, affinity chromatography and more recently, adherence of cells transfected with individual integrin α-subunits. The recognition sites within laminin-1 for the integrins were mapped to distinct regions and domains using defined proteolytic laminin fragments or more recently, using fragments expressed recombinantly. The most detailed investigations were carried out with laminin-1 from the murine EHS tumor.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aumailley M, Gimond C, Rousselle P. 6. Integrin-mediated cellular interactions with laminins. In: Ekblom P, Timpl R, eds. The Laminins. Harwood Academic Publishers 1996: 127–158.

    Google Scholar 

  2. Sonnenberg A, Moderman PW, Hogervorst F. Laminin receptor on platelets is the integrin VLA-6. Nature 1988; 336: 387–489.

    Article  Google Scholar 

  3. Aumailley M, Timpl R, Sonnenberg A. Antibody to integrin a6 subunit specifically inhibits cell-binding to laminin fragment 8. Exp Cell Res 1990; 188: 55–60.

    Article  PubMed  CAS  Google Scholar 

  4. Sonnenberg A, Linders CJT, Moderman PW et al. Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that a6ß1 but not a6134 functions as a major receptor for fragment E8. J Cell Biol 1990; 110: 2145–2155.

    Article  PubMed  CAS  Google Scholar 

  5. Hall DE, Reichardt LF, Crowley E et al. The 0(31 and a6ß1 integrin heterodimers mediate cell attachment to distinct sites on laminin. J Cell Biol 1990; 110: 2175–2184.

    Article  PubMed  CAS  Google Scholar 

  6. Deutzmann R, Aumailley M, Wiedemann H et al. Cell adhesion, spreading and neunte stimulation by laminin fragment E8 depends on maintenance of secondary and tertiary structure in its rod and globular domain. Eur J Biochem 1990; 191: 513–522.

    Article  PubMed  CAS  Google Scholar 

  7. Aumailley M, Nurcombe V, Edgar D et al. The cellular interactions of laminin fragments. Cell adhesion correlates with two fragment-specific high affinity binding sites. J Biol Chem 1987; 262: 11532–11538.

    PubMed  CAS  Google Scholar 

  8. Goodman SL, Deutzmann R, von der Mark K. Two distinct cell-binding domains in

    Google Scholar 

  9. laminin can independently promote non-neuronal cell adhesion and spreading. J Cell Biol 1987; 105: 589–598.

    Article  Google Scholar 

  10. Edgar D, Timpl R, Thoenen H. The heparin-binding domain of laminin is responsible for its effects on neunte outgrowth and neuronal survival. EMBO J 1984; 3: 1463–1468.

    CAS  Google Scholar 

  11. Goodman SL, Risse G, von der Mark K. The E8 subfragment of laminin promotes locomotion of myoblasts over extracellular matrix. J Cell Biol 1989; 109: 799–809.

    Article  PubMed  CAS  Google Scholar 

  12. Sung U, O’Rear JJ, Yurchenco PD. Cell and heparin binding in the distal long arm of laminin: identification of active and cryptic sites with recombinant and hybrid glycoprotein. J Cell Biol 1993; 123: 1255–1268.

    Article  PubMed  CAS  Google Scholar 

  13. Tashiro K, Sephel GC, Weeks B et al. A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neunte outgrowth. J Biol Chem 1989; 264: 16174–16182.

    PubMed  CAS  Google Scholar 

  14. Lotz MM, Korzelius CA, Mercurio AM. Human colon carcinoma cells use multiple receptor to adhere to laminin: involvement of a6134 and a2I31 integrins. Cell Regul 1990; 2: 249–257.

    Google Scholar 

  15. Lee EC, Lotz MM, Steele GD et al. The integrin a6134 is a laminin receptor. J Cell Biol 1992; 117: 671–678.

    Article  PubMed  CAS  Google Scholar 

  16. Niessen CM, Hogervorst F, Jaspars LH et al. The a6134 integrin is a receptor for both laminin and kalinin. Exp Cell Res 1994; 211: 360–367.

    Article  PubMed  CAS  Google Scholar 

  17. Sonnenberg A, Gehlsen, K, Aumailley M et al. Isolation of u6131 integrins from platelets and adherent cells by affinity chromatography on mouse laminin fragment E8 and human laminin pepsin fragment. Exp Cell Res 1991; 197: 234–244.

    Article  PubMed  CAS  Google Scholar 

  18. Rousselle P, Aumailley M. Kalinin is more efficient than laminin in promoting adhesion of primary keratinocytes and some other epithelial cells and has a different requirement for integrin receptors. J Cell Biol 1994; 125: 205–214.

    Article  PubMed  CAS  Google Scholar 

  19. Kramer RH, Vu MP, Cheng Y-F et al. Laminin-binding integrin a7111; functional characterization and expression in normal and malignant melanocytes. Cell Regul 1991; 2: 805–817.

    PubMed  CAS  Google Scholar 

  20. Song WK, Wang W, Foster RF et al. H36-a7 is a novel integrin a-chain that is developmentally regulated during skeletal myogenesis. J Cell Biol 1992; 117: 643–657.

    Article  PubMed  CAS  Google Scholar 

  21. Song WK, Wang W, Sato H et al. Expression of a7 integrin cytoplasmic domains during skeletal muscle development: alternate forms, conformational change, and homologies with serine/threonine kinases and tyrosine phosphatases. J Cell Sci 1993; 106: 1139–1152.

    PubMed  CAS  Google Scholar 

  22. Gu M, Wang W, Song WK et al. Selective modulation of the interaction of 07131 integrin with fibronectin and laminin by L-14 lectin during skeletal muscle differentiation. J Cell Sci 1994; 107: 175–181.

    PubMed  CAS  Google Scholar 

  23. Ziober BL, Vu MP, Waleh N et al. Alternative extracellular and cytoplasmic domains of the integrin a7 subunit are differentially expressed during development. J Biol Chem. 1993; 268: 26773–26783.

    PubMed  CAS  Google Scholar 

  24. Von der Mark H, Dürr J, Sonnenberg A et al. Skeletal myoblasts utilize a novel 131 series integrin and not a6131 for binding to the E8 and T8 fragments of laminin. J Biol Chem 1991;. 266: 23593–23601.

    Google Scholar 

  25. Echtermeyer F, Schober S, Pöschl E et al. Specific induction of cell motility on laminin by a7 integrin. J Biol Chem 1996; 271: 2071–2075.

    Article  PubMed  CAS  Google Scholar 

  26. Languino LR, Gehlsen KR, Wayner EA et al. Endothelial cells use 02(31 integrin as a laminin receptor. Cell Biol 1989; 109: 2455–2462.

    Article  CAS  Google Scholar 

  27. Elices MJ, Hemler ME. The human integrin VLA-2 is a collagen receptor on some cells and a collagen/laminin receptor on others. Proc Natl Acad Sci USA 1989; 86: 9906–9910.

    Article  PubMed  CAS  Google Scholar 

  28. Ignatius MJ, Large TH, Houde M et al. Molecular cloning of the rat integrin al subunit: a receptor for laminin and collagen. J Cell Biol 1990; 111: 709–720.

    Article  PubMed  CAS  Google Scholar 

  29. Tomaselli KJ, Hall DE, Flier LA et al. A neuronal cell line (PC12) expresses two 3–1 class integrin 01131 and 03131 that recognize different neunte outgrowth promoting domains in laminin. Neuron 1990; 5: 651–662.

    Article  PubMed  CAS  Google Scholar 

  30. Goodman SL, Aumailley M, von der Mark H. Multiple cell surface receptors for the short arms of laminin: a1ß1 integrin and RGD-dependent proteins mediate cell attachment only to domain III in murine tumor laminin. J Cell Biol 1991; 113: 931–941.

    Article  PubMed  CAS  Google Scholar 

  31. Pfaff M, Göhring W, Brown J et al. Binding of purified collagen receptors (a1131, a2131) and RGD-dependent integrins to laminins and laminin fragments. Eur J Biochem 1994; 225: 975–984.

    Article  PubMed  CAS  Google Scholar 

  32. Forsberg E, Paulsson M, Timpl R et al. Characterization of a laminin receptor on rat hepatocytes. J Biol Chem 1990; 265: 6376–6381.

    PubMed  CAS  Google Scholar 

  33. Rossino P, Gavazzi I, Timpl R et al. Nerve growth factor induces increased expression of a laminin-binding integrin in rat pheochromocytoma PC12 cells. Exp Cell Res 1990; 189: 100–108.

    Article  PubMed  CAS  Google Scholar 

  34. Aumailley M, Gerl M, Sonnenberg A et al. Identification of the Arg-Gly-Asp sequence in laminin A chain as a latent cell-binding site being exposed in fragment P1. FEBS Lett 1990; 262: 82–86.

    Article  PubMed  CAS  Google Scholar 

  35. Brown JC, Wiedemann H, Timpl R. Protein binding and cell adhesion properties of two laminin isoforms (AmBleB2e, AmBlsB2e) from human placenta. J Cell Sci 1994; 107: 329–338.

    PubMed  CAS  Google Scholar 

  36. Delwel GO, Hogervorst F, Kuikman L et al. Expression and function of cytoplasmic variants of the integrin a6 subunit in transfected K562 cells: activation-dependent adhesion and interaction with isoforms of laminin. J Biol Chem 1993; 268: 25865–25875.

    PubMed  CAS  Google Scholar 

  37. Delwel GO, de Melker AA, Hovervorst F et al. Distinct and overlapping ligand specificities of the a3A(31 and a6A(31 integrins. recognition of laminin isoforms. Mol Biol Cell 1994; 5: 203–215.

    PubMed  CAS  Google Scholar 

  38. Fietzek PP, Kühn K. The primary structure of collagen. Int Rev Connective Tissue Res 1976; 7: 1–60.

    CAS  Google Scholar 

  39. Salem G, Traub W. Conformational implications of aminoacid sequence regularities in collagen. FEBS Lett 1975; 51: 94–99.

    Article  PubMed  CAS  Google Scholar 

  40. Brazel D, Pollner R, Oberbäumer I et al. Human basement membrane collagen (type IV). The aminoacid sequence of the a2(IV) chain and its comparison with the al(IV) chain reveals deletions in the al(IV) chain. Eur J Biochem 1988; 172: 35–42.

    Article  PubMed  CAS  Google Scholar 

  41. Rich A, Crick FCH. The molecular structure of collagen. J Mol Biol 1961; 3: 483–506.

    Article  PubMed  CAS  Google Scholar 

  42. Brodsky B, Shah NK. The triple helix motif in proteins. FASEB J 1995; 9: 1537–1546.

    CAS  Google Scholar 

  43. Main AL, Harvey TS, Baron M et al. The three-dimensional structure of the 10th type III module of fibronecti an insight into RGD mediated interactions. Cell 1992; 71: 671–678.

    Article  PubMed  CAS  Google Scholar 

  44. Aumailley M, Mann K, von der Mark H et al. Cell attachment properties of collagen type VI and Arg-Gly-Asp dependent binding to its a2(VI) and a3(VI) chains. Exp Cell Res 1989; 181: 463–474.

    Article  PubMed  CAS  Google Scholar 

  45. Pfaff M, Aumailley M, Specks U et al. Integrin and Arg-Gly-Asp dependence of cell-adhesion to the native and unfolded triple helix of collagen type VI. Exp Cell Res 1993; 206: 167–176.

    Article  PubMed  CAS  Google Scholar 

  46. Kiihn K, Eble J. The structural bases of integrin-ligand interaction. Trends Cell Biol 1994; 4: 256–261.

    Article  Google Scholar 

  47. Pfaff M, Göhring W, Brown JC et al. Binding of purified collagen receptors (al(31, u2ß1) and RGD-dependent integrins to laminins and laminin fragments. bur J Biochem 1994; 225: 975–984.

    CAS  Google Scholar 

  48. Santoro SA, Zutter MM. The u2131 integrin: a collagen receptor on platelets and other cells. Thromb Haemos 1995; 74: 813–821.

    CAS  Google Scholar 

  49. Williams MJ, Du X, Loftus JC et al. Platelet adhesion receptors. Seminars in Cell Biol 1995; 6: 305–314.

    Article  CAS  Google Scholar 

  50. Morton LF, Hargreaves PG, Farndale RW et al. Integrin u2131-independent activation of platelets by simple collagen-like peptides: collagen tertiary (triple-helical) and quaternary (polymeric) structures are sufficient alone for a2131- independent platelet reactivity. Biochem J 1995; 306: 337–344.

    PubMed  CAS  Google Scholar 

  51. Peterson DM, Stathopoulos NA, Giorgion TD et al. Shear-induced platelet aggregation requires von Willebrand factor and platelet membrane glycoproteins Ib and IIbIIIa. Blood 1987; 69: 625–628.

    PubMed  CAS  Google Scholar 

  52. Ikeda Y, Handa M, Kawano K et al. The role of von Willebrand factor and fibrinogen in platelet aggregation under varying shear stress. J Clin Invest 1991; 87: 1234–1240.

    Article  PubMed  CAS  Google Scholar 

  53. Savage B, Shattil SJ, Ruggeri ZM. Modulation of platelet function through adhesion receptors: a dual role for glycoprotein lib-IIIa (integrin alb(33) mediated by fibrinogen and glycoprotein Ib-von Willebrand factor. J Biol Chem 1992; 267: 11300–11306.

    PubMed  CAS  Google Scholar 

  54. Fauvel F, Legrand YJ, Bentz 1–1 et al. Platelet-collagen interaction: adhesion of human blood platelets to purified (CB4) peptide from type Ill collagen. Thromb Res 1978; 12: 841–850.

    Article  PubMed  CAS  Google Scholar 

  55. Fauvel F, Legand YJ, Kühn K et al. Platelet adhesion to type III collagen: involvement of a sequence of nine aminoacids from al(III)CB4 peptide. Thromb Res 1979; 16: 269–273.

    Article  PubMed  CAS  Google Scholar 

  56. Staatz WD, Walsh JJ, Pexton T et al. The (x2(31 integrin cell surface collagen receptor binds to the al(I)-CB3 peptide of collagen. J Biol Chem 1990; 265: 4778–4781.

    PubMed  CAS  Google Scholar 

  57. Staatz WD, Fok KF, Zutter MM et al. Identification of a tetrapeptide: recognition sequence for the u2(31 integrin in collagen. J Biol Chem 1991; 12: 7363–7367.

    Google Scholar 

  58. Morton LF, Peachy AR, Zijenah LS et al. Conformation-dependent platelet adhesion to collagen involving integrin a231-mediated and other mechanisms: multiple a2(31-recognition sites in collagen type I. Biochem J 1994; 299: 791–797.

    CAS  Google Scholar 

  59. Morton LF, Zijenah LS, Coller BS et al. Collagen-platelet interaction x2131 inte- grin-dependent and other mechanisms. Throm llaemos 1991; 65: 679–679.

    Google Scholar 

  60. Gullberg D, Terracio L, Borg TK et al. Identification of integrin-like matrix receptors with affinity for interstitial collagens. J Biol Chem 1989; 264: 12686–12694.

    PubMed  CAS  Google Scholar 

  61. Gullberg D, Turner DC, Borg TK et al. Different ß1-integrin collagen receptors on rat hepatocytes and cardiac fibroblasts. Exp Cell Res 1990; 190: 254–264.

    Article  PubMed  CAS  Google Scholar 

  62. Gullberg D, Gehlsen KR, Turner DC et al. Analysis of a1ß1, n2131 and (x3(31 integrins in cell-collagen interactions: identification of conformation dependent al131 binding sites in collagen type I. EMBO J 1992; 11: 3865–3873.

    CAS  Google Scholar 

  63. Santoro SA, Zutter MM, Wu JE et al. Analysis of collagen receptors. Methods Enzym 1994; 215: 117–183.

    Google Scholar 

  64. Sakakibara K, Inouza K, Shido K et al. Synthesis of (Pro-Hyp-Gly). of defined molecular weights. Evidence for the stabilization of collagen triple helix by hydroxyproline. Biochim Biophys Acta 1973; 303: 198–202.

    Article  PubMed  CAS  Google Scholar 

  65. Morton LF, McCulloch IY, Barnes MJ. Platelet aggregation by a collagen-like synthetic peptide. Thromb Res 1993; 72: 367–372.

    Article  PubMed  CAS  Google Scholar 

  66. Palotie A, Peltonen L, Risteli L et al. Effects of the structural components of basement membranes on the attachment of teratocarcinoma-derived endodermal cells. Exp Cell Res 1983; 144: 31–37.

    Article  PubMed  CAS  Google Scholar 

  67. Murray JC, Stingl G, Kleinman HK et al. Epidermal cells adhere preferentially to type IV (basement membrane) collagen. J Cell Biol 1979; 80: 197–212.

    Article  PubMed  CAS  Google Scholar 

  68. Aumailley M, Timpl R. Attachment of cells to basement membrane collagen type IV. J Cell Biol 1986; 103: 1569–1575.

    Article  PubMed  CAS  Google Scholar 

  69. Dennis J, Waller C, Timpl R et al. Surface sialic acid reduces attachment of metastatic tumor cells to collagen type IV and fibronectin. Nature (Lond) 1982; 300: 274–276.

    Article  CAS  Google Scholar 

  70. Engvall E, Bell ML, Carlsson NLK et al. Nonhelical, fibronectin-binding basement membrane collagen from endodermal cell culture. Cell 1982; 29: 475–482.

    Article  PubMed  CAS  Google Scholar 

  71. Clyman RI, Turner DC, Kramer RH. An a1/ß1-like integrin receptor on rat aortic smooth muscle cells mediates adhesion to laminin and collagen types I and IV. Arteriosclerosis 1989; 10: 402–409.

    Google Scholar 

  72. Herbst TJ, McCarthy JB, Tsilibay EC et al. Differential effects of laminin. Intact type IV collagen and specific domains of type IV collagen on endothelial cell. Adhesion and Migration. J Cell Biol 1988; 106: 1365–1373.

    Article  PubMed  CAS  Google Scholar 

  73. Vandenberg P, Kern A, Ries A et al. Characterization of a type IV collagen major cell binding site with affinity to the alß1 and a2131 integrins. J Cell Biol 1991; 113: 1475–1483.

    Article  PubMed  CAS  Google Scholar 

  74. Kern A, Eble JA, Golbik R et al. Interaction of type IV collagen with the isolated integrins ON and a2131. Eur J Biochem 1993; 215: 151–159.

    Article  PubMed  CAS  Google Scholar 

  75. Eble JA, Golbik R, Mann K et al. The a1131 integrin recognition site of the basement membrane collagen molecule lal(IV)]2a2(1V). EMBO J 1993; 12: 4795–4802.

    CAS  Google Scholar 

  76. Chelberg MK, McCarthy JB, Skubitz APN et al. Characterization of a synthetic peptide from type IV collagen that promotes melanoma cells adhesion, spreading and motility. J Cell Biol 1990; 111: 262–270.

    Article  Google Scholar 

  77. Fields CG, Mickelson DJ, Drake SL et al. Melanoma cell adhesion and spreading activities of a synthetic 124-residue triple-helical “mini-collagen”. J Biol Chem 1993; 268: 14153–14160.

    PubMed  CAS  Google Scholar 

  78. Rao GHR, Fields CG, White JG et al. Promotion of human platelet adhesion and aggregation by a synthetic, triple-helical “mini-collagen”. J Biol Chem 1994; 269: 13899–13903.

    PubMed  CAS  Google Scholar 

  79. Wilke MS, Furcht LT. Human keratinocytes adhere to a unique heparin-binding peptide sequence within the triple helical region of type IV collagen. J Invest Dermatol 1990; 95: 264–270.

    Article  PubMed  CAS  Google Scholar 

  80. Miles AJ, Skubitz APN, Furcht LT et al. Promotion of cell adhesion by single-stranded and triple-helical peptide models of basement membrane collagen al(IV)531543. J Biol Chem 1994; 269: 30939–30945.

    PubMed  CAS  Google Scholar 

  81. Miles AJ, Knutson JR, Skubitz APN et al. A peptide model of basement membrane collagen al(IV)53l-543 binds the n3131 integrin. J Biol Chem 1995; 270: 29047–29050.

    Article  PubMed  CAS  Google Scholar 

  82. Maldonado BA, Furcht LT. Involvement of integrins with adhesion-promoting, heparin-binding peptides of type IV collagen in cultured human corneal epithelial cells. Investigative Ophthalmology and Visual Science 1995; 36: 364–372.

    PubMed  CAS  Google Scholar 

  83. Timpl R, Brown JC. The laminins. Matrix Biol 1994; 14: 275–281.

    Article  CAS  Google Scholar 

Download references

Authors
  1. Klaus Kühn
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Kühn, K. (1997). Conformation-Dependent Recognition Sites. In: Integrin-Ligand Interaction. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-4064-6_6

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-4064-6_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-4066-0

  • Online ISBN: 978-1-4757-4064-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation