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Construction of Xylose-Utilizing Cyanobacterial Chassis for Bioproduction Under Photomixotrophic Conditions

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Synthetic Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2760))

Abstract

Xylose is a major component of lignocellulose and the second most abundant sugar present in nature after glucose; it, therefore, has been considered to be a promising renewable resource for the production of biofuels and chemicals. However, no natural cyanobacterial strain is known capable of utilizing xylose. Here, we take the fast-growing cyanobacteria Synechococcus elongatus UTEX 2973 as an example to develop the synthetic biology-based methodology of constructing a new xylose-utilizing cyanobacterial chassis with increased acetyl-CoA for bioproduction.

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Acknowledgments

This chapter was supported by grants from the National Key Research and Development Program of China (Grant Nos. 2019YFA0904600, 2018YFA0903000, 2018YFA0903600, and 2020YFA0906800) and the National Natural Science Foundation of China (Grant Nos. 31901016, 31770035, 31972931, 32270091, and 21621004).

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Author notes

  1. Xinyu Song and Yue Ju contributed equally with all other contributors.

Authors and Affiliations

  1. Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, People’s Republic of China

    Xinyu Song, Yue Ju, Lei Chen & Weiwen Zhang

  2. Key Laboratory of Systems Bioengineering and Frontier Science Center of Synthetic Biology, The Ministry of Education of China, Tianjin University, Tianjin, People’s Republic of China

    Xinyu Song, Yue Ju, Lei Chen & Weiwen Zhang

  3. Center for Biosafety Research and Strategy, Tianjin University, Tianjin, People’s Republic of China

    Xinyu Song & Weiwen Zhang

Authors
  1. Xinyu Song
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  2. Yue Ju
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  3. Lei Chen
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  4. Weiwen Zhang
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Corresponding author

Correspondence to Weiwen Zhang .

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Editors and Affiliations

  1. Agilent Technologies, Inc, La Jolla, CA, USA

    Jeffrey Carl Braman

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Song, X., Ju, Y., Chen, L., Zhang, W. (2024). Construction of Xylose-Utilizing Cyanobacterial Chassis for Bioproduction Under Photomixotrophic Conditions. In: Braman, J.C. (eds) Synthetic Biology. Methods in Molecular Biology, vol 2760. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3658-9_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3658-9_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3657-2

  • Online ISBN: 978-1-0716-3658-9

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