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Fetal Thymic Organ Culture and Negative Selection

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T-Cell Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2580))

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Abstract

Negative selection removes potentially harmful T cell precursors from the conventional T cell pool. This process can involve the induction of apoptosis, anergy, receptor editing, or deviation into a regulatory T cell lineage. As such, this process is essential for the health of an organism through its contribution to central and peripheral tolerance. While a great deal is known about the process, the precise mechanisms that regulate these various forms of negative selection are not clear. Numerous models exist with the potential to address these questions in vitro and in vivo. This chapter describes fetal thymic organ culture methods designed to analyze the signals that determine these unique cell fates.

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Authors and Affiliations

  1. Department of Molecular Microbiology and Immunology, NextGen Precision Health, University of Missouri, Columbia, MO, USA

    Emma Teixeiro & Mark A. Daniels

Authors
  1. Emma Teixeiro
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  2. Mark A. Daniels
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Corresponding author

Correspondence to Mark A. Daniels .

Editor information

Editors and Affiliations

  1. Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

    Remy Bosselut

  2. Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

    Melanie S. Vacchio

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© 2023 This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply

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Teixeiro, E., Daniels, M.A. (2023). Fetal Thymic Organ Culture and Negative Selection. In: Bosselut, R., Vacchio, M.S. (eds) T-Cell Development. Methods in Molecular Biology, vol 2580. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2740-2_18

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  • DOI: https://doi.org/10.1007/978-1-0716-2740-2_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2739-6

  • Online ISBN: 978-1-0716-2740-2

  • eBook Packages: Springer Protocols

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