Changes in Antioxidative Compounds and Enzymes in Small-Leaved Linden (Tilia cordata Mill.) in Response to Mistletoe (Viscum album L.) Infestation
Abstract
:1. Introduction
2. Results
2.1. Effect of Mistletoe Infestation on the Relative Water Content and the Content of Chlorophylls in Linden Leaves
2.2. Effect of Mistletoe Infestation on Oxidative Stress Parameters in Linden Leaves
2.3. Effect of Mistletoe Infestation on Non-Enzymatic Antioxidants in Linden Leaves
2.4. Effect of Mistletoe Infestation on Antioxidative Enzyme Activity in Linden Leaves
2.5. Interaction between Relative Water Content, Chlorophyll Content, Oxidative Stress, and Antioxidative Response of Linden Trees to Mistletoe Infestation
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Determination of Relative Water Content
4.3. Determination of Chlorophyll Content
4.4. Determination of Oxidative Stress Parameters
4.5. Determination of Non-Enzymatic Antioxidant Content
4.6. Determination of Antioxidative Enzyme Activity
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatment | RWC, % | Chlorophyll a, mg g−1 DW | Chlorophyll b, mg g−1 DW | Total Chlorophyll, mg g−1 DW | Chlorophyll a/b Ratio |
---|---|---|---|---|---|
C | 89.2 ± 0.7 a | 2.25 ± 0.03 a | 1.56 ± 0.02 a | 3.80 ± 0.04 a | 1.44 ± 0.01 a |
NI | 89.1 ± 1.0 a | 2.52 ± 0.04 a | 1.51 ± 0.05 a | 3.75 ± 0.05 a | 1.51 ± 0.03 a |
LI | 83.6 ± 0.5 b | 1.79 ± 0.06 b | 1.23 ± 0.03 b | 3.03 ± 0.07 b | 1.45 ± 0.02 a |
HI | 77.8 ± 0.9 c | 1.52 ± 0.05 c | 1.14 ± 0.03 b | 2.66 ± 0.06 c | 1.33 ± 0.03 b |
Treatment | Electrolyte Leakage, % | MDA, nmol g−1 DW | H2O2, nmol g−1 DW |
---|---|---|---|
C | 12.4 ± 0.7 c | 93.6 ± 2.0 c | 2.24 ± 0.03 a |
NI | 15.8 ± 0.6 c | 99.4 ± 3.1 c | 2.25 ± 0.03 a |
LI | 23.8 ± 1.3 b | 137.8 ± 4.4 b | 1.79 ± 0.05 b |
HI | 30.8 ± 1.4 a | 183.8 ± 3.6 a | 1.52 ± 0.6 c |
Treatment | Proline, µmol g−1 DW | GSH, µmol g−1 DW | GSSG, µmol g−1 DW | AsA, µg g−1 DW | DHA, µg g−1 DW | DKGA, µg g−1 DW | TPC, mg g−1 DW |
---|---|---|---|---|---|---|---|
C | 155.2 ± 5.1 c | 53.8 ± 1.6 a | 4.10 ± 0.11 b | 111.6 ± 3.0 c | 44.2 ± 1.4 c | 36.8 ± 2.0 c | 9.48 ± 0.39 b |
NI | 219.0 ± 5.5 b | 45.6 ± 1.2 b | 4.12 ± 0.14 b | 218.6 ± 5.8 a | 63.8 ± 2.7 b | 38.4 ± 1.5 c | 9.20 ± 0.41 b |
LI | 239.8 ±5.4 b | 32.8 ± 1.4 c | 5.70 ± 0.10 a | 230.6 ± 3.7 a | 79.4 ± 1.5 a | 61.4 ± 2.3 b | 12.92 ± 0.86 a |
HI | 321.4 ± 4.7 a | 23.0 ± 1.5 d | 5.84 ± 0.12 a | 178.2 ± 5.2 b | 84.6 ± 1.5 a | 110.1 ± 4.2 a | 5.44 ± 0.29 c |
Treatment | SOD, U mg−1 Protein | CAT, nmol H2O2 mg−1 Protein min−1 | APX, µmol AsA mg−1 Protein min−1 | MDAR, µmol NADPH mg−1 Protein min−1 | DHAR, µmol AsA mg−1 Protein min−1 | GPX, µmol GSH mg−1 Protein min−1 | GR, µmol NADPH mg−1 Protein min−1 | POD, µmol Guaiacol mg−1 Protein min−1 |
---|---|---|---|---|---|---|---|---|
C | 4.46 ± 0.05 c | 131.1 ± 2.9 c | 14.1 ± 1.0 d | 3.47 ± 0.06 c | 1.96 ± 0.03 b | 1.33 ± 0.03 d | 2.22 ± 0.04 b | 0.58 ± 0.04 c |
NI | 4.44 ± 0.04 c | 134.2 ± 2.8 c | 24.1 ± 1.3 c | 3.49 ± 0.04 c | 2.77 ± 0.06 a | 2.18 ± 0.03 c | 2.21 ± 0.03 b | 0.68 ± 0.01 bc |
LI | 7.57 ± 0.06 a | 227.8 ± 3.9 a | 36.6 ± 1.4 b | 4.50 ± 0.05 a | 2.87 ± 0.04 a | 3.30 ± 0.05 b | 2.29 ± 0.02 b | 1.48 ± 0.05 a |
HI | 7.46 ± 0.11 b | 174.0 ± 2.7 b | 48.5 ± 1.8 a | 3.97 ± 0.04 b | 1.49 ± 0.05 c | 3.53 ±0.04 a | 2.68 ± 0.04 a | 0.80 ± 0.02 b |
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Skrypnik, L.; Maslennikov, P.; Feduraev, P.; Pungin, A.; Belov, N. Changes in Antioxidative Compounds and Enzymes in Small-Leaved Linden (Tilia cordata Mill.) in Response to Mistletoe (Viscum album L.) Infestation. Plants 2021, 10, 1871. https://doi.org/10.3390/plants10091871
Skrypnik L, Maslennikov P, Feduraev P, Pungin A, Belov N. Changes in Antioxidative Compounds and Enzymes in Small-Leaved Linden (Tilia cordata Mill.) in Response to Mistletoe (Viscum album L.) Infestation. Plants. 2021; 10(9):1871. https://doi.org/10.3390/plants10091871
Chicago/Turabian StyleSkrypnik, Liubov, Pavel Maslennikov, Pavel Feduraev, Artem Pungin, and Nikolay Belov. 2021. "Changes in Antioxidative Compounds and Enzymes in Small-Leaved Linden (Tilia cordata Mill.) in Response to Mistletoe (Viscum album L.) Infestation" Plants 10, no. 9: 1871. https://doi.org/10.3390/plants10091871