Deficiency of Acute-Phase Serum Amyloid A Exacerbates Sepsis-Induced Mortality and Lung Injury in Mice
Abstract
:1. Introduction
2. Results
2.1. Whole-Body SAA Deficiency Aggravates Sepsis-Induced Mortality
2.2. SAA Is Dispensable for Endotoxin and Bacterial Clearance or Systemic Proinflammatory Responses
2.3. Deficiency of SAA Does Not Change Plasma HDL-Cholesterol (HDL-C) Levels in Mice during Sepsis
2.4. Deficiency of SAA Exacerbates Sepsis-Induced Kidney and Lung Injury in Mice
2.5. Deficiency of SAA Increases the Expression of Genes Involved in Leukocyte Chemotaxis in Lung Tissues during Sepsis
2.6. Deficiency of SAA Increases the Accumulation of Neutrophils in the Lungs of Septic Mice and SAA Attenuates In Vitro Neutrophil Transmigration
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Mouse Models of Sepsis
4.3. Cytokine, Glucose, Endotoxin, and Tissue Injury Marker Analysis
4.4. Analysis of Bacterial Burden
4.5. Plasma SAA, Total Cholesterol, and Triglyceride Measurements
4.6. RNA Isolation and Quantitative RT-PCR
4.7. Histology
4.8. Myeloperoxidase (MPO) Activity
4.9. Neutrophil Transmigration Assay
4.10. Lung Function Measurements
4.11. RNA Sequencing and Analysis
4.12. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ji, A.; Trumbauer, A.C.; Noffsinger, V.P.; Meredith, L.W.; Dong, B.; Wang, Q.; Guo, L.; Li, X.; De Beer, F.C.; Webb, N.R.; et al. Deficiency of Acute-Phase Serum Amyloid A Exacerbates Sepsis-Induced Mortality and Lung Injury in Mice. Int. J. Mol. Sci. 2023, 24, 17501. https://doi.org/10.3390/ijms242417501
Ji A, Trumbauer AC, Noffsinger VP, Meredith LW, Dong B, Wang Q, Guo L, Li X, De Beer FC, Webb NR, et al. Deficiency of Acute-Phase Serum Amyloid A Exacerbates Sepsis-Induced Mortality and Lung Injury in Mice. International Journal of Molecular Sciences. 2023; 24(24):17501. https://doi.org/10.3390/ijms242417501
Chicago/Turabian StyleJi, Ailing, Andrea C. Trumbauer, Victoria P. Noffsinger, Luke W. Meredith, Brittany Dong, Qian Wang, Ling Guo, Xiangan Li, Frederick C. De Beer, Nancy R. Webb, and et al. 2023. "Deficiency of Acute-Phase Serum Amyloid A Exacerbates Sepsis-Induced Mortality and Lung Injury in Mice" International Journal of Molecular Sciences 24, no. 24: 17501. https://doi.org/10.3390/ijms242417501