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Variability in chlorophyll fluorescence spectra of eggplant fruit grown under different light environments: a case study

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abstract

The main goal of the present work was to clarify physiological strategies in plants whose chloroplasts were developed under different light environments. The specific objective was to elucidate the influence of the spectral distribution of light on the chlorophyll fluorescence ratio and on photosynthetic parameters. To achieve this purpose, three species of eggplant fruit (black, purple and white striped and white) were used as a case study and their chlorophyll fluorescence was analyzed in detail. Spectra of the non-variable fluorescence in each part of the fruit were corrected for distortions by light reabsorption processes using a physical model. The main conclusion of this work was that the corrected fluorescence ratio was dependent on the contribution of each photosystem to the fluorescence and consequently on the environmental lighting conditions, becoming higher when illumination was rich in long wavelengths. Variable chlorophyll fluorescence, similar to that observed from plant leaves, was detected for the pulp of the black eggplant, for the pulp of the purple and white striped eggplant and for the intact fruit of the black eggplant. The maximum quantum efficiency of photosystem II. in the light-adapted state (F’v/F’m), the quantum efficiency of photosystem II (ϕ5PS), and the photochemical and non-photochemical quenching coefficients (qP. and qNP/NPQ. respectively) were determined in each case. The results could be explained very interestingly, in relation with the proportion of exciting light reaching each photosystem (I. and II). The photochemical parameters obtained from variable chlorophyll fluorescence, allowed us to monitor non-destructively the physiological state of the black fruit during storage under both chilled or room-temperature conditions.

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Abbreviations

PSI:

Photosystem I

PSII:

Photosystem II

F 0 :

Ground chlorophyll fluorescence triggered by the measuring light in the dark-adapted state

F’ 0 :

Ground chlorophyll fluorescence measured after application of 735 nm far-red light

F m :

Maximum chlorophyll fluorescence caused by application of a saturating light pulse on dark-adapted leaves

F m :

Maximum chlorophyll fluorescence caused by application of a saturating light pulse in the light-adapted state of the leaf

F′ v :

Variable fluorescence in the light-adapted state (F’m - F′0

F′v/F′0:

Maximum quantum yield of PS2 photochemistry (alternative expression more sensitive than

(ϕPSII:

Quantum efficiency of photosystem II

qP:

Photochemical quenching coefficient

NPQ and qNP:

Non-photochemical quenching coefficients

F′v/Fm:

Maximum quantum yield of photosynthesis of leaves adapted to light or antennae efficiency of PSII

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Authors and Affiliations

  1. Facultad de Ciencias Exactas y Naturales, INQUIMAE, Universidad de Buenos Aires, CONICET, Buenos Aires, Argentina

    Brian Ospina Calvo, Tamara L. Parapugna & M. Gabriela Lagorio

  2. Dpto. de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria., Pabell’n II, 1er piso, C1428EHA, Argentina

    Brian Ospina Calvo, Tamara L. Parapugna & M. Gabriela Lagorio

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  1. Brian Ospina Calvo
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Correspondence to M. Gabriela Lagorio.

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Calvo, B.O., Parapugna, T.L. & Lagorio, M.G. Variability in chlorophyll fluorescence spectra of eggplant fruit grown under different light environments: a case study. Photochem Photobiol Sci 16, 711–720 (2017). https://doi.org/10.1039/c6pp00475j

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